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viral vector

  • Autonomously Replicating RNAs of Bungowannah Pestivirus: E<sup>RNS</sup> Is Not Essential for the Generation of Infectious Particles
    Genome Replication and Regulation of Viral Gene Expression
    Autonomously Replicating RNAs of Bungowannah Pestivirus: ERNS Is Not Essential for the Generation of Infectious Particles

    The proteins NPRO and ERNS are unique for the genus Pestivirus, but only NPRO has been demonstrated to be nonessential for in vitro growth. While this was also speculated for ERNS, it has always been previously shown that pestivirus replicons with deletions of the structural proteins ERNS, E1, or E2 did not produce any infectious progeny virus in susceptible host...

    Anja Dalmann, Ilona Reimann, Kerstin Wernike, Martin Beer
  • Comparative Evaluation of the Vaccine Efficacies of Three Adenovirus-Based Vector Types in the Friend Retrovirus Infection Model
    Vaccines and Antiviral Agents
    Comparative Evaluation of the Vaccine Efficacies of Three Adenovirus-Based Vector Types in the Friend Retrovirus Infection Model

    AdV-based vectors are important tools for the development of vaccines against a wide range of pathogens. While AdV vectors are generally considered safe and highly effective, their application can be severely impaired by preexisting immunity due to the widespread seroprevalence of some AdV types. The characterization of different AdV types with regard to immunogenicity and efficacy in challenge models is of great importance for the...

    Camilla Patrizia Hrycak, Sonja Windmann, Wibke Bayer
  • Novel Modified Vaccinia Virus Ankara Vector Expressing Anti-apoptotic Gene <em>B13R</em> Delays Apoptosis and Enhances Humoral Responses
    Vaccines and Antiviral Agents
    Novel Modified Vaccinia Virus Ankara Vector Expressing Anti-apoptotic Gene B13R Delays Apoptosis and Enhances Humoral Responses

    MVA is an attractive viral vector for vaccine development due to its safety and immunogenicity in multiple species and humans even under conditions of immunodeficiency. Here, to further improve the immunogenicity of MVA, we developed a novel vector, MVA-B13R, by replacing the fragmented anti-apoptotic genes 181R/182R with a functional version derived from vaccinia virus, B13R. Our results show that...

    Lynette S. Chea, Linda S. Wyatt, Sailaja Gangadhara, Bernard Moss, Rama R. Amara
  • The <em>135</em> Gene of Goatpox Virus Encodes an Inhibitor of NF-κB and Apoptosis and May Serve as an Improved Insertion Site To Generate Vectored Live Vaccine
    Pathogenesis and Immunity
    The 135 Gene of Goatpox Virus Encodes an Inhibitor of NF-κB and Apoptosis and May Serve as an Improved Insertion Site To Generate Vectored Live Vaccine
    Minmin Zhang, Yirui Sun, Weiye Chen, Zhigao Bu
  • Gene Delivery
    Cellular Antisilencing Elements Support Transgene Expression from Herpes Simplex Virus Vectors in the Absence of Immediate Early Gene Expression

    Gene therapy has now entered a phase of development in which a growing number of recessive single gene defects can be successfully treated by vector-mediated introduction of a wild-type copy of the gene into the appropriate tissue. However, many disease conditions, such as neurodegeneration, cancer, and inflammatory processes, are more complex, requiring either multiple gene corrections or provision of coordinated gene activities to...

    Fang Han, Yoshitaka Miyagawa, Gianluca Verlengia, Selene Ingusci, Marie Soukupova, Michele Simonato, Joseph C. Glorioso, Justus B. Cohen
  • Vaccines and Antiviral Agents
    A Respiratory Syncytial Virus Vaccine Vectored by a Stable Chimeric and Replication-Deficient Sendai Virus Protects Mice without Inducing Enhanced Disease
    Marian Alexander Wiegand, Gianni Gori-Savellini, Claudia Gandolfo, Guido Papa, Christine Kaufmann, Eva Felder, Alessandro Ginori, Maria Giulia Disanto, Donatella Spina, Maria Grazia Cusi
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