transcription factors
Genome Replication and Regulation of Viral Gene ExpressionGenetic Variability of Long Terminal Repeat Region between HIV-2 Groups Impacts Transcriptional ActivityOver 1 million patients are infected with HIV-2, which is often described as an attenuated retroviral infection. Patients frequently have undetectable viremia and evolve at more slowly toward AIDS than HIV-1-infected patients. Several studies have reported a smaller viral reservoir in peripheral blood mononuclear cells in HIV-2-infected patients than in HIV-1-infected patients, while others have found similar sizes of reservoirs but a...
Transformation and OncogenesisTAF Family Proteins and MEF2C Are Essential for Epstein-Barr Virus Super-Enhancer ActivitySEs play critical roles in cancer development. Since SEs assemble much bigger protein complexes on enhancers than typical enhancers (TEs), they are more sensitive than TEs to perturbations. Understanding the protein composition of SEs that are linked to key oncogenes may identify novel therapeutic targets. A genome-wide CRISPR screen specifically identified proteins essential for MYC ESE activity but not simian virus 40 (SV40) enhancer...
Genome Replication and Regulation of Viral Gene ExpressionA Noncanonical Basic Motif of Epstein-Barr Virus ZEBRA Protein Facilitates Recognition of Methylated DNA, High-Affinity DNA Binding, and Lytic ActivationThe binding of ZEBRA to methylated and unmethylated viral DNA triggers activation of the EBV lytic cycle, leading to viral replication and, in some patients, cancer development. Our work thoroughly examines how ZEBRA uses a previously unrecognized basic motif to bind nonmethylated and methylated DNA targets, leading to viral lytic activation. Our findings show that two different positively charged motifs, including the canonical BZIP...
Genetic Diversity and EvolutionRNA Polymerase Mutations Selected during Experimental Evolution Enhance Replication of a Hybrid Vaccinia Virus with an Intermediate Transcription Factor Subunit Replaced by the Myxoma Virus OrthologPrevious studies demonstrated the experimental evolution of vaccinia virus (VACV) following deletion of a host range gene important for evasion of host immune defenses. We have extended experimental evolution to essential genes that cannot be deleted but could be replaced by a divergent orthologous gene from another poxvirus. Replacement of a VACV transcription factor gene with one from a distantly related poxvirus led to decreased...
- Genome Replication and Regulation of Viral Gene ExpressionIdentification and Functional Characterization of Phosphorylation Sites of the Human Papillomavirus 31 E8^E2 Protein
- Genome Replication and Regulation of Viral Gene ExpressionIdentification of Vaccinia Virus Replisome and Transcriptome Proteins by Isolation of Proteins on Nascent DNA Coupled with Mass Spectrometry
- Virus-Cell InteractionsTRIM25 Is Required for the Antiviral Activity of Zinc Finger Antiviral Protein