reassortment
Genome Replication and Regulation of Viral Gene ExpressionGeneration of Infectious Recombinant Human Rotaviruses from Just 11 Cloned cDNAs Encoding the Rotavirus GenomeHuman group A rotavirus (HuRVA) is a leading pathogen causing severe diarrhea in young children worldwide. In this paper, we describe the generation of recombinant HuRVA (strain KU) from only 11 cloned cDNAs encoding the HuRVA genome by reverse genetics. The growth properties of the recombinant HuRVA were similar to those of the parental RVA, providing a powerful tool for better understanding of HuRVA replication and pathogenesis....
Structure and AssemblyComponents of the Reovirus Capsid Differentially Contribute to StabilityNonenveloped and enveloped viruses are exposed to the environment during transmission to a new host. Protein-protein and/or protein-lipid interactions stabilize the particle and protect the viral genome. Mammalian orthoreovirus (reovirus) is composed of two concentric, protein shells. The μ1 and σ3 proteins form the outer capsid; contacts between neighboring subunits are thought to confer resistance to inactivating agents. We further...
Structure and AssemblyProtein Mismatches Caused by Reassortment Influence Functions of the Reovirus CapsidCells coinfected with viruses that possess a multipartite or segmented genome reassort to produce progeny viruses that contain a combination of gene segments from each parent. Reassortment places new pairs of genes together, generating viruses in which mismatched proteins must function together. To test if such forced pairing of proteins that form the virus shell or capsid alters the function of the particle, we investigated properties...
- Genetic Diversity and EvolutionDivergent Human-Origin Influenza Viruses Detected in Australian Swine Populations
We describe the evolutionary origins and antigenic properties of influenza A viruses isolated from two separate Australian swine populations from 2012 to 2016, showing that these viruses are distinct from each other and from those isolated from swine globally. Whole-genome sequencing of virus isolates revealed a high genotypic diversity that had been generated exclusively through the introduction and establishment of human influenza...
- Genetic Diversity and Evolution | SpotlightInfluenza A Virus Reassortment Is Limited by Anatomical Compartmentalization following Coinfection via Distinct Routes
- Pathogenesis and ImmunityZoonotic Risk, Pathogenesis, and Transmission of Avian-Origin H3N2 Canine Influenza Virus
- Genetic Diversity and EvolutionSeasonal H3N2 and 2009 Pandemic H1N1 Influenza A Viruses Reassort Efficiently but Produce Attenuated Progeny
- Genetic Diversity and EvolutionGroup A Rotaviruses in Chinese Bats: Genetic Composition, Serology, and Evidence for Bat-to-Human Transmission and Reassortment
