mutagenesis
Virus-Cell InteractionsEndoplasmic Reticulum (ER) Reorganization and Intracellular Retention of CD58 Are Functionally Independent Properties of the Human Cytomegalovirus ER-Resident Glycoprotein UL148In myriad examples, viral gene products cause striking effects on cells, such as activation of stress responses. It can be challenging to decipher how such effects contribute to the biological roles of the proteins. The HCMV glycoprotein UL148 retains CD58 within the ER, thereby preventing it from reaching the cell surface, where it functions to stimulate cell-mediated antiviral responses. Intriguingly, UL148 also triggers the formation...
Genetic Diversity and Evolution | SpotlightDeep Mutational Scanning Comprehensively Maps How Zika Envelope Protein Mutations Affect Viral Growth and Antibody EscapeZika virus has recently been shown to be associated with severe birth defects. The virus’s E protein mediates its ability to infect cells and is also the primary target of the antibodies that are elicited by natural infection and vaccines that are being developed against the virus. Therefore, determining the effects of mutations to this protein is important for understanding its function, its susceptibility to vaccine-mediated immunity...
- Virus-Cell InteractionsFunctional Analysis of the Dengue Virus Genome Using an Insertional Mutagenesis Screen
- Genome Replication and Regulation of Viral Gene ExpressionGenome-Wide Mutagenesis of Dengue Virus Reveals Plasticity of the NS1 Protein and Enables Generation of Infectious Tagged Reporter Viruses
- Genome Replication and Regulation of Viral Gene ExpressionDifferential Requirement of Human Cytomegalovirus UL112-113 Protein Isoforms for Viral Replication
- Genetic Diversity and Evolution | SpotlightTransposon Mutagenesis of the Zika Virus Genome Highlights Regions Essential for RNA Replication and Restricted for Immune Evasion
