live-cell imaging
Virus-Cell Interactions | SpotlightHIV Infection Stabilizes Macrophage-T Cell Interactions To Promote Cell-Cell HIV SpreadOnce HIV enters the lymphoid organs, exponential viral replication in T cells ensues. Given the densely packed nature of these tissues, where infected and uninfected cells are in nearly constant contact with one another, efficient HIV spread is thought to occur through cell-cell contacts in vivo. However, this has not been formally demonstrated. In this study, we performed live-cell imaging studies within a 3-dimensional space...
Virus-Cell InteractionsVisualization of Calcium Ion Loss from Rotavirus during Cell EntryNonenveloped viruses penetrate into the cytosol of the cells that they infect by disrupting the membrane of an intracellular compartment. The molecular mechanisms of membrane disruption remain largely undefined. Functional reconstitution of infectious rotavirus particles (TLPs) from RNA-containing core particles (DLPs) and the outer layer proteins that deliver them into a cell makes these important pediatric pathogens particularly good...
- Virus-Cell InteractionsDifferential Disruption of Nucleocytoplasmic Trafficking Pathways by Rhinovirus 2A Proteases