List of 75 K15-interacting proteins identified by LC-MS/MS after lytic reactivationa

Gene(s)Protein(s)Log2 FE, expt 1Log2 FE, expt 2
PSMD1126S proteasome non-ATPase regulatory subunit 117.72.5
PSMC126S protease regulatory subunit 42.12.6
LIMA1LIM domain and actin-binding protein 15.26.6
CTTNSrc substrate cortactin7.58.6
HADHATrifunctional enzyme subunit alpha, mitochondrial; long-chain enoyl-CoA hydratase; long chain 3-hydroxyacyl-CoA dehydrogenase2.42.9
MYO1BUnconventional myosin-Ib5.45.6
ARF4, ARF5ADP-ribosylation factor 4, ADP-ribosylation factor 52.93.2
MYO1CUnconventional myosin-Ic3.44.6
BASP1Brain acid soluble protein 17.15.8
KPRPKeratinocyte proline-rich protein3.04.3
CLTAClathrin light chain A6.59.0
G3BP2Ras GTPase-activating protein-binding protein 23.84.1
GNB1, GNB2,GNB4Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1, guanine nucleotide-binding protein subunit beta-2, guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-47.33.8
CKAP4Cytoskeleton-associated protein 43.95.4
DLSTDihydrolipoyllysine residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex, mitochondrial5.34.5
PPP1R12AProtein phosphatase 1 regulatory subunit 12A2.97.2
AP2B1AP-2 complex subunit beta8.04.3
RBM14RNA-binding protein 144.52.4
DNAJB11DnaJ homologue subfamily B member 112.92.1
ACTR2Actin-related protein 27.38.1
PDHA1Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial3.34.7
HADHBTrifunctional enzyme subunit beta, mitochondrial, 3-ketoacyl-CoA thiolase2.34.1
FXR1, FXR2Fragile X mental retardation syndrome-related protein 1, fragile X mental retardation syndrome-related protein 24.13.2
ACTR3Actin-related protein 37.65.0
ARPC4Actin-related protein 2/3 complex subunit 47.28.7
VAT1Synaptic vesicle membrane protein VAT-1 homologue3.23.0
MYO1DUnconventional myosin-Id5.74.8
H1F0Histone H1.0, N-terminally processed7.74.7
MRPS2728S ribosomal protein S27, mitochondrial2.12.8
PAWRPRKC apoptosis WT1 regulator protein6.16.0
AP2A1AP-2 complex subunit alpha-15.53.9
AP2A2AP-2 complex subunit alpha-26.46.1
PIK3C2APhosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha6.96.6
PICALMPhosphatidylinositol-binding clathrin assembly protein8.25.0
ACOT9Acyl-coenzyme A thioesterase 9, mitochondrial6.55.7
ARPC3Actin-related protein 2/3 complex subunit 33.77.6
DECR12,4-Dienoyl-CoA reductase, mitochondrial6.84.2
HSDL2Hydroxysteroid dehydrogenase-like protein 23.83.4
FHL2Four-and-a-half LIM domains protein 27.15.4
CYR61Protein CYR615.05.3
REPS1RalBP1-associated Eps domain-containing protein 15.03.6
RABEP1Rab GTPase-binding effector protein 15.07.7
DAB2Disabled homologue 27.95.9
LEPRE1Prolyl 3-hydroxylase 12.02.3
SNX9Sorting nexin, sorting nexin-95.74.5
FRYLProtein furry homologue-like3.93.2
ARPC5Actin-related protein 2/3 complex subunit 55.23.0
STOMErythrocyte band 7 integral membrane protein2.74.4
CD59CD59 glycoprotein2.85.6
MYL3, MYL1Myosin light chain 3; myosin light chain 1/3, skeletal muscle isoform6.33.4
IKBIPInhibitor of nuclear factor κB kinase-interacting protein2.63.3
PBDC1Protein PBDC13.52.8
GIPC1PDZ domain-containing protein GIPC13.16.3
SCCPDHSaccharopine dehydrogenase-like oxidoreductase4.54.4
NAA50N-Alpha-acetyltransferase 502.53.8
TGM2Protein-glutamine gamma-glutamyltransferase 25.03.8
FGF2Fibroblast growth factor, fibroblast growth factor 22.57.5
FECHFerrochelatase, mitochondrial6.56.0
ISG15Ubiquitin-like protein ISG156.44.8
ARPC5LActin-related protein 2/3 complex subunit 5-like protein5.12.5
CD109CD109 antigen5.65.2
SQRDLSulfide:quinone oxidoreductase, mitochondrial4.24.0
FCHO2F-BAR domain-only protein 22.72.7
MX2Interferon-induced GTP-binding protein Mx26.15.3
SERPINE1Plasminogen activator inhibitor 17.14.4
  • a The pK15-interacting proteins identified from cells treated with a reactivation cocktail (RTA plus SB) was processed similarly to those in Table 1, and the resulting list of proteins is shown here. Proteins annotated to be involved in the endocytic pathway by using the DAVID bioinformatics tool are in boldface type. FE, fold enrichment.