TABLE 1

HI titers of ferret antisera against mutant MN/10 and BJ/92 viruses

VirusAntigenic site(s)HI titera of ferret sera
MN/10BJ/92
wt
    MN/10 4535
    BJ/92 5640
MN/10 reassortantsb
    Q122NA45310
    S124DA45310
    S135GA32010
    R140KA3205
    122/124/135/140A32020
    Y155HB2265
    N156KB1605
    L157SB32010
    N158EB22614
    K189RB3205
    K193SB1605
    155/156B22614
    155/158B11314
    155/189B1135
    155/193B16028
    156/189B405
    156/193B8020
    158/189B16014
    158/193B16020
    189/193B16020
    155/156/158B11340
    155/156/189B5728
    155/156/193B5757
    155/158/189B5720
    155/158/193B8057
    155/189/193B16028
    156/158/189B5720
    156/158/193B11340
    156/189/193B160113
    158/189/193B11380
    155/156/158/189B2828
    155/156/158/193B57113
    155/156/189/193B40113
    155/158/189/193B5780
    156/158/189/193B57226
    155/156/158/189/193B28226
    156/157/158/189/193B80453
    135/155/156/158/189/193A and B28453
    155/156/157/158/189/193B28453
BJ/92 reassortantsc
    BJ156/158/189/193B4020
    BJ156/157/158/189/193B16040
  • a Geometric mean titer from two independent assays; a value of 5 was assigned to titers of <10 for calculation.

  • b Reassortant viruses possessing the HA and NA genes of MN/10 virus and the internal genes of PR8, each containing a single residue change (e.g., Q122N) or a combination of changes (e.g., 122, 124, 135, and 140; abbreviated 122/124/135/140) to that/those present in BJ/92 HA.

  • c Reassortant viruses containing the HA and NA genes of BJ/92 and the internal genes of PR8, with residues at the indicated positions mutated to those in MN/10 HA.