TABLE 1

Flow cytometry analysis of apoptosis of W12ti cells upon depletion of SRSF2

Treatment% of cells positive for annexin V-propidium iodide ± SDa
Live compartmentEarly apoptosisLate apoptosis
Control siRNA31.2 ± 5.820.2 ± 5.120.4 ± 2.1
SRSF2 siRNA8.0 ± 2.755.1 ± 5.836.4 ± 2.1
E6 siRNA7.0 ± 2.328.1 ± 3.549.7 ± 8.9
UVB6.1 ± 4.445.0 ± 4.344.3 ± 5.7
  • a Percentage of cells positive for annexin V-propidium iodide upon treatment with control siRNA, siRNA against SRSF2 or E6, or UVB (500 J/m2 24 h prior to harvesting). The numbers are the means and standard deviations from the mean from three separate experiments.