TABLE 2

Apoptosis-related p53, Bcl2/cytochrome c, and ISG family genes measured by absolute quantitative RT-PCR

Gene designationApoptotic impactForward primer sequence (5′–3′)Reverse primer sequence (5′–3′)Median gene expression (IQR), expression unitsP value, HIV vs HIV+
HIV, n = 19HIV+, n = 30
Rb1Antiapoptotic (22, 27, 61)GCC TCT CGT CAG GCT TGA GTCA TCT AGG TCA ACT CGT GCA A2.559 (1.729–8.421)7.563 (5.633–9.302)0.0100
MDM2GAA TCA TCG GAC TCA GGT ACA TCTCT GTC TCA CTA ATT GCT CTC CT1.359 (0.7060–3.316)1.634 (1.192–2.660)NSa
Bcl2Antiapoptotic (14, 46, 69)TTG CCA GCC GGA ACC TAT GCGA AGG CGA CCA GCA ATG ATA0.1108 (0.08957–0.3244)0.3394 (0.09620–0.7771)NS
Bcl-wAntiapoptotic (14, 46, 69)GCG GAG TTC ACA GCT CTA TACAAA AGG CCC CTA CAG TTA CCA1.052 (0.6412–1.314)0.6553 (0.4295–0.9256)0.0169
Mcl-1Antiapoptotic (14, 46, 69)TGC TTC GGA AAC TGG ACA TCATAG CCA CAA AGG CAC CAA AAG0.8846 (0.5637–1.242)0.8340 (0.5756–1.101)NS
BaxAntiapoptotic (14, 46, 69)CCC GAG AGG TCT TTT TCC GAGCCA GCC CAT GAT GGT TCT GAT0.6709 (0.4939–1.030)0.8039 (0.6534–0.9233)NS
Bak1Antiapoptotic (14, 46, 69)GTT TTC CGC AGC TAC GTT TTTGCA GAG GTA AGG TGA CCA TCT C1.868 (1.338–3.304)2.201 (1.759–2.906)NS
BikAntiapoptotic (14, 46, 69)GAC CTG GAC CCT ATG GAG GACCCT CAG TCT GGT CGT AGA TGA0.6191 (0.3536–1.256)0.6983 (0.4991–1.149)NS
CycSProapoptotic (33, 47)CTT TGG GCG GAA GAC AGG TCTTA TTG GCG GCT GTG TAA GAG0.8745 (0.4069–1.406)1.313 (0.9209–1.627)0.0392
Mx1GTT TCC GAA GTG GAC ATC GCACCA TTC AGT AAT AGA GGG TGG GA0.1703 (0.1163–0.3839)1.140 (0.4724–2.399)<0.0001
IFI6Antiapoptotic (12, 63)GGT CTG CGA TCC TGA ATG GGTCA CTA TCG AGA TAC TTG TGG GT0.8497 (0.4130–1.969)5.150 (2.086–12.72)0.0002
IFI27Proapoptotic (56)TGG CTC TGC CGT AGT TTT GTCC TCC AAT CAC AAC TGT AGC A0.3359 (0.1101–0.8334)158.7 (10.34–533.5)<0.0001
IFITM2Proapoptotic (17)ATG AAC CAC ATT GTG CAA ACC TCCC AGC ATA GCC ACT TCC T1.798 (1.167–2.780)3.128 (2.105–4.960)0.0015
  • a NS, not significant.