TABLE 3

Mouse MAbs used for antibody binding assay

MAbIsotypeHI titeraMutation in HA of escape mutants
HA13IgG1<10NDb
HA20IgG110,240K160T or N165Kc
HA25IgG15,120Y159D
HA59IgG1<10ND
HB1IgG2a5,120155YN- -YK160d
HB27IgG2a10,240Y159D or 155YN- -YK160
HB67IgG2a320G142R or S143L
HE42IgG12,560N145K or N158D
Mouse serum 640ND
  • a Measured with 1 mg/ml of each MAb. MN/10 antiserum collected from the mouse used for generation of the HA-specific MAbs was used as a positive control.

  • b ND, not done.

  • c These mutations are present in different mutants selected by the same MAb.

  • d The mutant possesses a deletion of 2 amino acids, either 156NL157 or 157LN158, from the wt sequence 155YNLNYK160.