TABLE 1

Recombinant viruses and lentiviral vectors used in the study

Virus nameaParental virus/vectorbCharacteristicsc
KJ6TMEV (DA1)Capsid adapted to infect L929 cells
TM659KJ6Zn finger mutation in L protein capsid adapted to infect L929 cells
KJ7KJ6GFP-coding sequence replacing codons 5–67 of L capsid adapted to infect L929 cells
SD1TMEV (GDVII)Zn finger mutation in L protein; efficient at infecting in vitro-cultured neurons
KJ26TMEV (GDVII)GFP-coding sequence replacing codons 1–67 of L; efficient at infecting in vitro-cultured neurons
VSV-GFPVSVGFP-coding sequence
pTM941pCCLsinLentiviral vector (PGK-MCS-IRES-eGFP)
pTM942pCCLsinLentiviral vector (PGK-MCS-IRES-mCherry)
pMK44pTM942ApoL9 expression
pMK46pTM942Dhx58 expression
pMK42pTM942Tor3a expression
pMK50pTM942SP100 expression
pMK51pTM942Irgm2 expression
pMK53pTM942Ifi202b expression
pMK56pTM942Ifi203 expression
pMK43pTM942Lgals9 expression
pMK84pLKO.1mCherry-coding region replacing puroR
pMK74pMK84shRNA targeting Apol9b coding region
pMK75pMK84shRNA targeting Apol9b 3′ UTR
  • a Plasmids used to produce the listed viruses by reverse genetics have a “p” before the virus name (e.g., pKJ6 for virus KJ6).

  • b pCCLsin, pCCLsin.PPT.hPGK.GFP.pre.

  • c L, leader protein of Theiler's virus; PGK, human phosphoglycerate kinase promoter; MCS, multicloning site; eGFP, enhanced GFP; puroR, puromycin resistance-coding region.