TABLE 4

DNA and protein divergence in ORFs across the EEHV1A, EEHV1B, and EEHV6 genomesa

Gene locusEEHV1A(Kimba) coordinatesbProtein sizebNucleotide level divergence (%)cAmino acid level divergence (%)cChimeric domain
1A-1B1A-61B-61A-1B1A-61B-6
U39(gB)73959–76511836211620141113CD-I
U38/(POL)(76544–79043)(832)5171641212CD-I
U33(83628–84428)(266)1.322210.31918
U28(RRA)99358–997608011.11415088
U27.5(RRB)99804–1007093021.414140.31717
U27(PPF)100960–1021864081.816160.566
U45.7(ORF-J)102168–102775168261625321433CD-II
U46(gN)102759–10371396291628206.218CD-II
U47(gO)103075–103713212351635381137CD-II
U48(gH)(105363–105903)(179)311728332137CD-II
U48.5(TK)105835–106908356131618162120CD-II
U49106910–1076082323.615150.41213
U50(PAC2)(107427–108083)(220)4.023233.12021
U51(vGPCR1)(109398–110239)(286)4.222233.31417
U57(MCP)(115577–117866)(748)2.717171.277
U60(TERex3)123601–124175(194)2.912130d0d0d
U62124231–124477880.81313099
U71(MyrTeg)132954–133241968252592326
U72(gM)(133320–133614)(97)4.413143.39.27.2
U73(OBP)(134615–135415)(277)3.014141.96.06.7
U76(POR)(139998–141295)(433)1.9131304.24.2
U77(HEL)(141294–141864)(190)2.812130.57.07.5
U77.5(ORF-M)143988–145502503161619131316CD-III
U80.5(ORF-N)145642–145959106Del31DelDel29DelCD-III
U81(UDG)146027–146980317291930291930CD-III
U82(gL)146946–147860304311830332241CD-III
U82.5(ORF-O)(147700–147910)(78)331631341837CD-III
U85.5(ORF-K)(152200–153350)(380)1.726260.82525
U86.5(ORF-L)(155324–158615)(1084)2.411110.61818
  • a The rows in the table shown in bold type indicate ORFs that are highly variable between EEHV1A and EEHV1B.

  • b The EEHV1A coordinates are based on Ling et al. (13). The values shown in parentheses indicate that an incomplete ORF or shorter region was used.

  • c The divergence between EEHV1A and EEHV1B (1A-1B), EEHV1A and EEHV6 (1A-6), and EEHV1B and EEHV6 (1B-6) is shown. Del, ORF-N is absent (deleted) in all EEHV1B strains.

  • d All three TER proteins are identical or only 1 aa different over this 194-aa segment.