TABLE 1

Primers used for cloning

PrimerSequence (5′ → 3′)aRestriction site created
ORF27FGTTGTTCATATGAGCCAGAATACTTCCAGCGCCGTCNdeI
ORF27RAACAACGCGGCCGCTGCCGCCACCTCTTCGCGATAGNotI
ORF65FGTTGTTCATATGAGCGCCATCACCGCTCAGNdeI
ORF65RTCACTCGAGCGCCAACCTCTCGACCTGTTTCXhoI
1F_ecoRGTTGAATTCGTAACCAATGCTCCGATGCGEcoRI
1R_bamHGTTGGATCCCTCCTATCGTCCGCCCAGBamHI
2F_bamHGTTGGATCCGTAACCAATGCTCCGATGCGBamHI
2R_ecoRGTTGAATTCCTCCTATCGTCCGCCCAGEcoRI
attLfGTAGCGGGCCAAGATCATTG
attLrCGGCTCTAGGGGGAAGTCG
attRfCCCGAAGTAAGCAGGTAGACAC
attRrGTAGACGAGGCCTTCACCCTTC
CcrMfCGAGTAAGCGTATTTGCGAGTTG
CcrMrGGCGGAACTGGTTGATCAG
CcrM_NdeGTTCATATGTCTTCAGTTGTTTCGCTTGCNdeI
CcrM_XhoGTTCTCGAGGTTCAGTTTTGCCAGATCATTTCGXhoI
  • a The restriction enzyme site is indicted in bold.