TABLE 1

DENV3-specific epitopes identified in this study

Restriction classEpitopeSequenceT cell response (no. of SFC/106 T cells)HLA binding (IC50, nM)Conservation between virus strains (%)Reference(s)
S221D3S5CX
A*0101E451–459TTEAILPEY4293156100
NS11090–1099RSCTLPPLRY3055.910010022
A*0201C57–66FLAIPPTAGV8683.68010022
C103–112SLCLMMILPA2445.75010015
C106–114LMMILPAAL1,6796.65610015
M250–259ILALFLAHYI1,5956.35010015
M254–263FLAHYIGTSL1,3634.850100
M268–276VIFILLMLV60737567100
E580–589YAMCTNTFVL6583350100
E631–639RLITANPVV4182078100
E727–735ALFSGVSWV1,086207810015
NS1854–862ILWENNIKL2,2382.767100
NS1987–996KLEKASLIEV488778010039
NS2A1164–1172VLFTFVLLL1,3631045100
NS2A1202–1211YLALIATFKI1,089107010015
NS2A1271–1280YQLWTALVSL1,1221.74010015
NS2B1355–1363VMAVGLVSI442217810015
NS2B1444–1453VLLKTALLIV203235010047
NS31832–1840FAGKTVWFV415118910039, 48
NS31876–1884KLNDWDFVV3331.87810040
NS32013–2022ELMRRGDLPV757229010015, 39
NS4A2150–2159TLLLLGLMIL990526010011
NS4A2205–2213IVLEFFMMV4691167100
NS4B2311–2320SLAAIANQAV8586.780100
NS52814–2822SMINGVVKL1,016147810015
B*0702E509–517TPTWNRKEL1,1932.856100
NS1814–822SPKRLATAI1,1861.56710015
NS11071–1079GPSLRTTTV1152.28910015
NS2A1290–1298TVAWRTATL4863.656100
NS2B1373–1382VPMAGPLVAG281158010015
NS2B1457–1465FPYSIPATL8021.067100
NS31648–1656EPDGPTPEL51229944100
NS31682–1690LPAIVREAI4946.510010011, 15, 22, 3941
NS31700–1709APTRVVAAEM2914.610010015, 22, 3941, 44, 48
NS31899–1907RVIDPRRCL2601468910015, 36
NS31925–1934MPVTAASAAQ69010728010015
NS32070–2078RPRWLDART402.17810015, 40, 48
NS4A2113–2121LAHRTRNAL953.356100
NS52860–2868RPMPGTRKV4612.74410015
B*4001NS2B1412–1421AEQTGVSHNL3626750100
NS31569–1577GEEVQVIAV293127810039
NS31598–1607GEIGAIALDF289407010015
NS32005–2014GESRKTFVEL7083.58010015, 40
DRB1*0101C101–115KTSLCLMMILPAALA82124010015
M268–282VIFILLMLVTPSMTM53537310015
NS1984–998GSWKLEKASLIEVKT236566710040
NS2B1359–1373GLVSILASSLLRNDV2412.180100
NS31692–1706RRLRTLILAPTRVVA1135.39310015, 3941, 44, 48
NS31742–1756TFTMRLLSPVRVPNY701.510010015, 22, 40
NS52967–2981RAIWYMWLGARYLEF586489310015