TABLE 3

Relative inoculation doses of wt and var viruses impact which genotypes emerge following coinfectiona

Ratio (wt/var), inoculum (PFU)No. of var parental/total parental isolatesb (P = 0.0026)No. of var segments/total segments in reassortant isolatesc (P = 0.0005)
1:12.5, 1 × 1050.890.50
1.000.84
1.000.63
0.850.66
0.670.67
    Avg0.880.66
1:7, 2 × 1060.890.68
1.000.76
1.000.68
1.000.63
1.000.78
    Avg0.980.71
1:1, 2 × 1060.570.43
0.700.56
0.000.52
0.330.48
0.570.46
    Avg0.440.49
  • a Data are derived from day 2 nasal washes of guinea pigs 11 to 15 shown in Fig. 2 (1:1 group) and the guinea pigs shown in Fig. 3. A Student t test was used to compare guinea pigs inoculated with 1:1 and 1:7 mixtures at the 2 × 106 PFU dose. The P values are indicated in the respective column headings.

  • b The number of var parental isolates, divided by the sum of wt + var parental isolates, is shown.

  • c The number of var gene segments included in reassortant genomes, divided by the sum of wt + var gene segments in reassortant genomes, is shown.