TABLE 1

Variation in the frequency of reassortant progeny over time after infection

Guinea pig no.Inoculum dose (PFU)aGenotypes of virus isolates (%)b
Day 1Day 2Day 3Day 6
RwtvarRwtvarRwtvarRwtvar
12 × 102NDNDND010000100001000
22 × 102NDNDND509550955095
32 × 102NDNDND010000100001000
42 × 102NDNDND010000100001000
52 × 102NDNDNDNDNDNDNDNDNDNDNDND
62 × 1030595520753833295905
72 × 10302971059528244801486
82 × 1030811924433350955095
92 × 1035108510405057331035650
102 × 10300100059524715243838
112 × 10648331967141963370NDNDND
122 × 1065743050153567330NDNDND
132 × 1066332565350453520NDNDND
142 × 10662335702010741610NDNDND
152 × 10665251061172278175NDNDND
  • a Inocula contained 50% wt and 50% var viruses (n = 36). The total dose for wt + var is indicated.

  • b n = 18 to 21. R, any reassortant genotype. ND, virus was not detected in nasal washings at these time points (limit of detection = 50 PFU/ml).