TABLE 3

Mutations in sequenced viral stocks

Mutation frequency in total viral population (%)No. of SNVs in virus:
Md5B40BAC-c1 p5Rep 1 p75Rep 1 p85Rep 2 p65Rep 3 p75
≤23323211636
3–10353552345
11–20291147
21–3014341
31–4022120
41–5004414
51–6004530
61–7016213
71–8016101
81–9005123
91–10062213
Total no. of mutations identified49a11810657103
  • a Six mutations in Md5B40BAC-c1 p5 were fixed at 100% in our viral stocks relative to the reference Md5B40BAC sequence. Considering that these mutations were present in the parental virus preparations, and therefore all serially passed replicates, these mutations were not considered in any further SNV analysis in the attenuated replicates.