TABLE 2

Mutational primers for generation of recombinant viruses

Recombinant virusPrimer namePrimer sequencea
Mut UL42-D207GMDV055 D207G-fTTGAAAGCTGAAGGAGGTTTTTATGCCGGAACGATTTGTGgTGTGATAAGTTTTGATATAGtagggataacagggtaatcgattt
MDV055 D207G-rTTGGACCATTGCGCTTCCATCTATATCAAAACTTATCACAcCACAAATCGTTCCGGCATAAgccagtgttacaaccaattaacc
Mut UL46-Q117RMDV059 Q117R-fACACCTGCGGTGGTAAAAGACTACTACACAGACTCGTATCgGCGCTATGTCTGTAAGCGGCtagggataacagggtaatcgattt
MDV059 Q117R-rATCAACACAAGGTATACGCCGCCGCTTACAGACATAGCGCcGATACGAGTCTGTGTAGTAGgccagtgttacaaccaattaacc
Mut UL5- I682RMDV017 I682R-fGATAGTTATGTCGATAATGTGAGTTCGAGAGGATGTGAGAgATTCATAAACAACATGCGAGtagggataacagggtaatcgattt
MDV017 I682R-rAGCAAGGGATAACATTCCCCCTCGCATGTTGTTTATGAATcTCTCACATCCTCTCGAACTCgccagtgttacaaccaattaacc
Revt UL5-R682IMDV017 revertant R682I-fGATAGTTATGTCGATAATGTGAGTTCGAGAGGATGTGAGAtATTCATAAACAACATGCGAGtagggataacagggtaatcgattt
MDV017 revertant R682I-rAGCAAGGGATAACATTCCCCCTCGCATGTTGTTTATGAATaTCTCACATCCTCTCGAACTCgccagtgttacaaccaattaacc
LORF2-PromoterRep2 LORF2/17359-fTCTCGCACAATCTATGCAGAAACAGATAATCTAGGGTGTGtGCGGTGCTTTGTACTTCCTAtagggataacagggtaatcgattt
Rep2 LORF2/17359-rTTTTTATAACTGATCCGACGTAGGAAGTACAAAGCACCGCaCACACCCTAGATTATCTGTTgccagtgttacaaccaattaacc
LORF2-IntronRep3 LORF2/17545-fTTTCCGTCGTGAATTTGTACGCCAAATTTTACAACGTGAGaTTATATTGTTTGAAATTTCAtagggataacagggtaatcgattt
Rep3 LORF2/17545-rACGATAAAGATAATATACATTGAAATTTCAAACAATATAAtCTCACGTTGTAAAATTTGGCgccagtgttacaaccaattaacc
  • a Nucleotides in uppercase denote the portion of the primer designed to target the MDV genome. The desired point mutation to be incorporated is designated by a bold, underlined lowercase letter. Nucleotides in lowercase indicate the portion of the primer annealing to the plasmid template region containing the kanamycin cassette.