TABLE 2

Relative RT-qPCR data from SGE treatments of mouse embryonic fibroblasts followed by infection with multiple dengue virus serotypes and isolatesa

IsolateResult by SGE levelP valuee
01.0
DENV1 Hawaiib (m)1.1 (± 0.0)4.5 (±0.3)<0.05
DENV2 TH-36 (m)0.9 (± 0.0)10.6 (±1.6)<0.05
DENV3 CAESc (tc)1.7 (± 0.2)14.2 (±0.4)<0.05
DENV4 H241 (tc)1.0 (± 0.0)51.3 (±3.5)<0.05
DENV2 NGC (tc)1.0 (± 0.1)12.9 (±0.3<0.05
DENV2 PLO46d (tc)1.2 (± 0.3)30.0 (±3.9)<0.05
DENV2 E124/E128 (tc)1.7 (± 0.4)1.9 (±0.3)NS
  • a Data represent averages from at least three independent in vitro SGE-mediated enhancement assays (see Materials and Methods). Untreated samples were set to 1.0. Student's t tests were performed to assess statistical significance.

  • b Mouse-adapted DENV types 1 and 2 were obtained from the ATCC as detailed in Materials and Methods.

  • c Tissue culture-adapted DENV types 2 to 4 were obtained from the ATCC and the Connecticut Agricultural Experiment Station as detailed in Materials and Methods.

  • d Stocks from infectious clones of tissue culture-adapted DENV type 2 PLO46 and E124/128 were obtained from Sujan Shresta.

  • e Data represent averages from three or more independent experiments and include standard errors of the means. Student's t tests were performed to assess statistical significance. NS, not significant.