Table 5

Specific infectivity of influenza A/Denmark/524/2009 (H1N1) virus in the presence and absence of T-705 and zanamivira

DrugConcn (μM)Infectious virus yield (log10 PFU/ml)bVirus RNA copy no. (log10 genome copies/ml)cSpecific infectivity (infectious virus yield × 10−3/genome copy no.)
ExtracellularIntracellularExtracellularIntracellularExtracellularIntracellular
None (mock treated)06.71 ± 0.036.84 ± 0.1510.04 ± 0.1410.06 ± 0.640.490 ± 0.1750.714 ± 0.600
T-705104.68 ± 0.08*5.29 ± 0.089.40 ± 0.029.70 ± 0.010.019 ± 0.004*0.039 ± 0.01*
100<1.0*1.24 ± 0.3*5.83 ± 0.15*7.19 ± 0.050.001 ± 0.000*0.001 ± 0.00*
Zanamivir105.17 ± 0.225.89 ± 0.048.80 ± 0.1110.30 ± 0.150.224 ± 0.0840.128 ± 0.110
1005.73 ± 0.085.72 ± 0.058.46 ± 0.119.57 ± 0.140.184 ± 0.0130.148 ± 0.050
  • a *, P < 0.01 in comparison to mock-treated samples (two-way ANOVA).

  • b Infectious virus in supernatants and cellular lysates was titrated by plaque assay in MDCK cells. Values are means ± SD from two independent experiments.

  • c Virus RNA copy number was determined by quantitative real-time PCR of the matrix gene. Values are means ± SD from three independent experiments.