Table 3

Effect of T-705 on infectious virus yield and viral RNA copy number during serial passage in MDCK cells inoculated with a low dose of seasonal influenza A (H1N1) virusa

H1N1 virusPassageInfectious virus yield (log10 PFU/ml)bViral RNA copy no. (log10 genome copies/ml)cSpecific infectivity (infectious virus yield/viral RNA copy no.)d
Mock treatmentT-705 treatmentMock treatmentT-705 treatmentMock treatmentT-705 treatment
A/Brisbane/59/200707.6 ± 0.18NAe9.3 ± 0.33NA0.8 ± 0.26NA
67.4 ± 0.106.7 ± 0.05**9.2 ± 0.019.0 ± 0.090.8 ± 0.060.7 ± 0.07
128.5 ± 0.024.6 ± 0.02**10.1 ± 0.058.4 ± 0.14*0.8 ± 0.040.5 ± 0.08
188.5 ± 0.164.3 ± 0.17**10.2 ± 0.098.7 ± 0.04*0.8 ± 0.130.5 ± 0.11
248.5 ± 0.03<1.0**8.5 ± 0.694.3 ± 0.17**1.0 ± 0.360.2 ± 0.17**
A/New Jersey/15/200707.1 ± 0.06NA9.7 ± 0.03NA0.7 ± 0.05NA
67.3 ± 0.066.7 ± 0.04**9.7 ± 0.049.6 ± 0.010.8 ± 0.050.7 ± 0.03
128.0 ±0.095.8 ± 0.04**9.9 ± 0.039.0 ± 0.12**0.8 ± 0.060.6 ± 0.08
187.9 ± 0.085.0 ± 0.01**9.9 ± 0.099.4 ± 0.020.8 ± 0.090.5 ± 0.02
248.3 ± 0.13<1.0**8.7 ± 0.683.8 ± 0.13**1.0 ± 0.410.3 ± 0.13**
  • a *, P < 0.01, or **, P < 0.0001, significant differences with respect to mock-treated samples by two-way ANOVA.

  • b Infectious virus was titrated by plaque assay in MDCK cells. Values are means ± SD from three independent experiments.

  • c Virus RNA copy number was determined by quantitative real-time RT-PCR of the matrix gene. Values are means ± SD from three independent experiments.

  • d Infectious virus yield × 10−3/genome copy number, determined 24 h p.i.

  • e NA, not applicable.