Table 2

Number of mutations observed in influenza A (H1N1) viruses after serial passage in MDCK cells treated with T-705

H1N1 virusPassageNo. of nucleotide changes ina:
T-705 treatedMock treated
G→AC→TA→GT→CT→AC→AG→CG→AC→TA→GT→CT→AC→AG→C
Seasonal viruses
    A/Brisbane/59/2007122222
249*8*2121112121
    A/New Jersey/15/2007122221
245*5*431123
2009 Pandemic viruses
    A/Denmark/524/200941
    A/Denmark/528/200941
  • a Whole virus genomes were sequenced. Shown is the number of nucleotide changes in a total of 7,749 nucleotide sequences in viruses from the supernatants of T-705-treated MDCK cells compared to those in mock-treated MDCK cells. −, no nucleotide changes detected; *, P < 0.05 compared to mock-treated samples (unpaired two-tailed t test).