Table 4

Neutralizing titers against recombinant MVs possessing the IC-H protein with various amino acid substitutions

Cell lineMutationInfectivityNeutralizing titera
B5 (I)E81 (I)E103 (vi)2F4 (vii)
B95aWild type (−)+7,5361,72710,23122,141
F483A+3,7681,72710,231<346
Y543S+1,8841,7272,558692
D505SNANANANA
R533ANANANANA
II-18Wild type (−)+3,76427,63120,46211,070
F483ANANANANA
Y543ANANANANA
D505S+1,88413,81520,462<5
R533A+1,88427,63120,462173
  • a Data represent neutralizing titers for 1 mg/ml of immunoglobulin. NA, not applicable.