Table 1

Oligonucleotides used in the construction of lentivirus vectors for double deletion (pLKO-shDP) and triple depletion (pLKO-shDPS)

PrimerSequence
dblsh.for15′-CCGCAATTGATCACCGGTTCGAAAAAGGAGTTGGATCTCTCAGAATCTCTTGAATTCTG-3′
dblsh.rev15′-CTTGGAGAAAAGCCTTGTTTGGAGTTGGATCTCTCAGAATTCAAGAGATTCTGAGA-3′
dblsh.for25′-GCCGACAATTGTCTAGACCCGGGATCCGACGCCGCCATCTCTA-3′
7SKXma.for5′-CCGGCCCCGGGCTGCAGTATTTAGCATGCCCCACCC-3′
7SKXba.rev5′-GGCCGTCTAGAGAGGTACCCAGGCGGCGCACAAGC-3′
shSp7SK.for5′-CGCCGCCTGGGTACCTCGTGAGCCTGTGATCAATAATTCAAGAGATTATT-3′
shSp7SK.rev5′-CCGTCTAGACGCGTAAAAAGTGAGCCTGTGATCAATAATCTCTTGAATT-3′
shSp7SK.for25′-AGCAAGCGGCCGCTGATCTTCAGA-3′