Table 3

Design and sequences of α-helical anti-HCV peptides

GroupNo.PeptideNo. of net chargeNo. of hydrophobic interactionsSequenceaIC50 (μM) for H77 HCVpp
Parental19GBVA10+55CWVRLGRYLLRRLKTPFT2
Hydrophobic group22GBVA10-1(L9K)+63CWVRLGRYKLRRLKTPFT>20
23GBVA10-2(L9S)+53CWVRLGRYSLRRLKTPFT18
24GBVA10-3(L9A)+53CWVRLGRYALRRLKTPFT2
25GBVA10-4(L9V)+55CWVRLGRYVLRRLKTPFT16
26GBVA10-5(G6A)+55CWVRLARYLLRRLKTPFT2.5
27GBVA10-6(G6V)+59CWVRLVRYLLRRLKTPFT1.25
28GBVA10-7(G6L)+59CWVRLLRYLLRRLKTPFT1.25
Helical group28GBVA10-7(G6L)+59CWVRLLRYLLRRLKTPFT1.25
29GBVA10-8(G6L/P16L)+510CWVRLLRYLLRRLKTLFT1.25
30GBVA10-9(P16L)+56CWVRLGRYLLRRLKTLFT0.16
31GBVA10-10(L9I)+55CWVRLGRYILRRLKTPFT10
32GBVA10-11(L9I/L13I)+55CWVRLGRYILRRIKTPFT0.63
33GBVA10-12(L5I/L9I/L13I)+55CWVRIGRYILRRIKTPFT>20
Electric group34GBVA10-13(C1K/Y8K/T15K/T18K)+95KWVRLGRKLLRRLKKPFK>20
35GBVA10-14(C1K/Y8K/T15K)+85KWVRLGRKLLRRLKKPFT>20
36GBVA10-15(Y8K/T15K)+75CWVRLGRKLLRRLKKPFT>20
37GBVA10-16(Y8K)+65CWVRLGRKLLRRLKTPFT>20
38GBVA10-17(C1E/Y8E/T15E)+25EWVRLGRELLRRLKEPFT>20
39GBVA10-18(C1E/Y8E/T15E/T18E)+15EWVRLGRELLRRLKEPFE>20
40GBVA10-19(Y8E/T15E)+35CWVRLGRELLRRLKEPFT>20
41GBVA10-20(Y8E)+45CWVRLGRELLRRLKTPFT>20
  • a Bold italic letters indicate the substituted amino acids of the parental peptide; all amino acids are l-amino acids.