Table 2

Dissociation and kinetic rate constants of antibody binding to A244 gp120 proteinsa

AntibodybRate constant and/or KdA244 gp120A244 gD Δ11 gp120E.A244 Δ11 gp120
A32ka (× 103 M−1 s−1)76.8 ± 11.4134 ± 14.0222.6 ± 20.4
(C1)kd (× 103 s−1)0.47 ± 0.050.13 ± 0.0170.15 ± 0.03
Kd (nM)6.25 ± 1.41.0 ± 0.220.67 ± 0.13
19bka (× 103 M−1 s−1)130.3 ± 10.5170.3 ± 8.5239.3 ± 19.8
(V3)kd (× 103 s−1)1.54 ± 0.0951.4 ± 0.081.56 ± 0.08
Kd (nM)11.8 ± 0.218.24 ± 0.236.54 ± 0.38
697-Dka (× 103 M−1s−1)4.9 ± 1.124.9 ± 5.626.75 ± 0.71
(V2)kd (× 103 s−1)7.0 ± 1.985.24 ± 0.545.18 ± 0.6
Kd (nM)1,465.3 ± 317217.6 ± 45.7156.7 ± 34.0
830Aka (× 103 M−1 s−1)21.8 ± 3.141.1 ± 1.959.9 ± 4.6
(V2)kd (× 103 s−1)0.22 ± 0.060.07 ± 0.0030.088 ± 0.01
Kd (nM)10.2 ± 3.61.7 ± 0.161.56 ± 0.09
2158ka (× 103 M−1 s−1)16.4 ± 0.9828.7 ± 1.036.5 ± 1.8
(V2)kd (× 103 s−1)0.19 ± 0.040.10 ± 0.030.13 ± 0.04
Kd (nM)11.2 ± 1.63.7 ± 0.93.68 ± 1.1
CH01ka (× 103 M−1 s−1)3.73 ± 1.637.2 ± 15.149.0 ± 5.4
(V1/V2)kd (× 103 s−1)4.38 ± 0.529.9 ± 2.815.6 ± 1.5
Kd (nM)1,639 ± 601277.8 ± 42317 ± 31.9
CH01-04 UA1 (V1/V2)ka (× 103 M−1 s−1)6.7 ± 2.516.7 ± 6.317.6 ± 0.5
kd (× 103 s−1)21.5 ± 0.619.2 ± 1.318.8 ± 0.5
Kd (nM)3,500 ± 12001,100 ± 2501,050 ± 220
CH01-04 UA2 (V1/V2)ka (× 103 M−1 s−1)4.4 ± 2.320.8 ± 10.127.3 ± 11.4
kd (× 103 s−1)13.9 ± 0.217.7 ± 0.626.2 ± 1.0
Kd (nM)3,200 ± 950870 ± 200940 ± 50
PG9ka (× 103 M−1 s−1)5.0 ± 3.511.5 ± 0.610.9 ± 0.9
(V1/V2)kd (× 103 s−1)1.1 ± 0.40.55 ± 0.030.57 ± 0.06
Kd (nM)183 ± 44.048.1 ± 0.1552.6 ± 2.9
VRC01ka (× 103 M−1 s−1)17.6 ± 0.5213.3 ± 0.579.7 ± 0.43
(CD4 bs)kd (× 103 s−1)0.28 ± 0.020.21 ± 0.060.39 ± 0.03
Kd (nM)15.7 ± 1.715.8 ± 3.936.7 ± 1.4
  • a Each of the rate constants and Kd values was derived from at least three measurements on individual flow cells of the same sensor chip or from binding data collected on different sensor chips. The mean and SD of rate constants (ka and kd) and Kd values are reported for each antibody binding to the three different forms of monomeric E.A244 gp120 proteins.

  • b Parentheses indicate gp120 region recognized by the MAbs.