Table 3

Associations of nucleotide substitutions in the EnhII/BCP/PC region of HBV genotypes B and C with cirrhosis and HCC

Hot spotOR (95% CI)c
Genotype BGenotype C
Cirrhosis patients (n = 44) vs ASCs (n = 77) plus CHB patients (n = 64)HCC patients (n = 93) vs HCC-free HBsAg-positive subjects (n = 185)Cirrhosis patients (n = 162) vs ASCs (n = 174) plus CHB patients (n = 196)HCC patients (n = 404) vs HCC-free HBsAg-positive subjects (n = 532)
G1652Aa3.37 (1.57–7.24)b0.23 (0.12–0.44)b0.52 (0.29–0.93)0.37 (0.23–0.59)b
C1653T1.78 (1.09–2.91)2.41 (1.75–3.32)b
T1673Ca1.98 (0.94–4.18)0.32 (0.17–0.60)b1.22 (0.76–1.95)0.30 (0.19–0.46)b
T1674C0.36 (0.10–1.29)0.41 (0.17–0.99)0.45 (0.26–0.79)1.65 (1.19–2.28)b
T1674G0.97 (0.51–1.86)1.95 (1.29–2.94)b
G1719T1.90 (0.89–4.07)0.46 (0.23–0.92)1.20 (0.82–1.77)2.43 (1.84–3.22)b
A1726C1.44 (0.70–2.95)0.47 (0.28–0.79)
T1727G2.45 (1.11–5.42)0.35 (0.17–0.73)
T1727Aa1.82 (1.05–3.14)0.24 (0.14–0.41)b
A1727G1.64 (1.02–2.62)1.32 (0.98–1.77)
G1730Ca1.82 (0.90–3.68)0.25 (0.13–0.47)b1.51 (0.94–2.42)0.24 (0.15–0.40)b
T1753C1.84 (1.07–3.15)3.06 (2.17–4.32)b
A1762T/G1764A2.80 (1.36–5.77)2.51 (1.50–4.21)b4.99 (3.25–7.64)b4.37 (3.24–5.87)b
G1799Ca1.48 (0.74–2.95)0.25 (0.13–0.47)b1.49 (0.94–2.36)0.36 (0.24–0.55)b
A1846T0.95 (0.43–2.11)1.42 (0.83–2.43)0.89 (0.57–1.38)1.61 (1.22–2.14)b
G1896A2.72 (1.30–5.72)1.56 (0.93–2.63)1.42 (0.95–2.13)1.91 (1.46–2.50)b
G1899A1.80 (0.59–5.55)2.53 (1.21–5.28)3.59 (1.77–7.27)b4.44 (2.91–6.76)b
  • a The wild-type nucleotide and the corresponding mutation at these hot spots exchange between genotype B and genotype C.

  • b The difference is still significant after the Bonferroni correction (cutoff P value = 0.0031).

  • c OR, odds ratio. − indicates that this site is not a hot spot in the given genotype. Boldface type indicates significant values.