Table 3

Oligonucleotides used in this study

OligonucleotideSequence (5′ → 3′)aPurpose
32H-attPATA CAG TTT GTC CTG CGG CTT GAGPCR for attR site
32H-attBGAC AAA GTC AGT CAG GCG TTT ACCPCR for attR site
32H-rep-CF2AAT GGG CAA ATG AAT TCG CTA TGA AAprep construction
32H-rep-CR1TGG TAA TTG CGT GTC GAC TGA Gprep construction
32H-ant-CF1TGT TTG CAT GGA GAA TTC GAG ATG Cpant construction
32H-ant-CR1GCC GCA GAG TCG ACC TTT TAT TTT TPant, Pant* construction
32H-ant*-CF2CAT GGA GAA TTC GAG ATG ACA ACG GPant* construction
32s-Pant-CF1TCA GTT GAG CTC GTC ATG TAA GpPant_H::lux and pPant_N::lux construction
32s-Pant-CR1GGT GAT ACT GCC ACT AGT TCT CpPant_H::lux and pPant_N::lux construction
32H-recE-lacZ-Red-FAGT AAG TCA CAA CTG GAT ATG GTG GCC AAG AAC CCT TCC CTG TAG GCT GGA GCT GCT TCGSR5188 and SR5189 construction
32H-recT-lacZ-Red-RTCT TTG TTG CAG GTG TGG CGC CGT GGC GCC ACG GTG GTG A AT TCC GGG GAT CCG TCG ACCSR5188 and SR5189 construction
32H-rep-HA-Red-FCTC ATT CAT AAA CTT CGT GTT TGA GCA GAA CAA AAG CAA GTA TCC GTA TGA TGT TCC TGA TTA TGC TAG CCT CTA ATG TAG GCT GGA GCT GCT TCGSR5192 and SR5197 construction
32H-rep-HA-Red-RACC GCC ATC GGG CAG GTA AAG CGT CAG AAT GGC AGG GGA TAT TCC GGG GAT CCG TCG ACCSR5192 and SR5197 construction
32H-ant-HA-Red-FTCT CGA TTA CTG TGC AGA ACA GTT ACG AAA ACA AAC CAC ATA TCC GTA TGA TGT TCC TGA TTA TGC TAG CCT CTA ATG TAG GCT GGA GCT GCT TCGSR5197 construction
32H-ant-HA-Red-RTGT CAT AGC ATG AAT GTG ACA TGT CAC GAG GCC GCA GAA GAT TCC GGG GAT CCG TCG ACCSR5197 construction
pKT25-32H-rep-CF1AAC TGC AGG GAT GAA AAG TAT TTA TGA CATpKT25-rep construction
pKT25-32H-rep-CR1CGG GAT CCT TAC TTG CTT TTG TTC TGpKT25-rep and pUT18C-rep construction
pUT18C-32H-ant-CF1AAC TGC AGG ATG CAA CGG CAG TAT CApUT18C-ant construction
pUT18C-32H-ant-CR1CGG GAT CCT TAT GTG GTT TGT TTT CGT AACpUT18C-ant and pKT25-ant construction
pKT25-32H-ant-CF1AAC TGC AGG GAT GCA ACG GCA GTA TCApKT25-ant construction
pUT18C-32H-rep-CF1AAC TGC AGG ATG AAA AGT ATT TAT GAC ATA AGpUT18C-rep construction
pHIS-LT2-lexA-CF2TAT ATA CAC CCC ATG GGC GGA ATG AAA GpHIS-LexA construction
pHIS-LT2-lexA-CR2ATT GCC GGA TCT CGA GTT ACA AGG AGpHIS-LexA construction
pHIS-32H-Rep-CF1AAT ACC ATG GCT ATG AAA AGT ATT TAT GAC ATA AGA CGCpHIS-Rep construction
pHIS-32H-Rep-CR1AAA GCT CGA GAA TGG CAG GGG ATT ACT TGCpHIS-Rep construction
pHIS-32H-Ant-CF4CAT AAG CCA TGG GGA TGC AAC GGC AGT ATC ACpHIS-Ant construction
pHIS-32H-Ant-CR3GCC GCA GAC TCG AGC TTT TAT TTT TCA TTA TGT GGpHIS-Ant construction
32s-APR-CF1CTT CAG TTG AGA CCG TCA TGPCR for APRH and APRN
32s-APR-CR1TAA GAT GTG AGT CCT CCA CCPCR for APRH and APRN
  • a Restriction enzyme sites and HA tag coding sequences are underlined and italicized, respectively. Bold case indicates the artificially inserted redundant nucleotide to generate the frameshift in the ant gene.