Table 1

Bacterial strains and bacteriophages used in this study

Strain or phageRelevant characteristicsaReference or source
Bacterial strains
    Salmonella enterica serovar Typhimurium
        LT2(c)Prophage-cured strain LT2; wild type; host for phage SPC32H and SPC32N13
        SR5003LT2(c) with ΔLT2gtrABC114
        SR5100ΔLT2gtrABC1 (32H); SR5003 lysogenized by SPC32HThis study
        SR5158LT2(c) with ΔLT2gtrABC1 ΔsulA ΔlexAThis study
        SR5176LT2(c) with ΔLT2gtrABC1 ΔsulA lexA(G85D); non-cleavable LexA mutantThis study
        SR5189ΔLT2gtrABC1 (32H recET::lacZ); SR5003 lysogenized by SPC32H recET::lacZThis study
        SR5188ΔLT2gtrABC1 ΔsulA lexA(G85D) (32H recET::lacZ); SR5176 lysogenized by SPC32H recET::lacZThis study
        SR5192ΔLT2gtrABC1(32H rep-HA); SR5003 lysogenized by SPC32H rep-HAThis study
        SR5197ΔLT2gtrABC1(32H rep-HA ant-HA); SR5003 lysogenized by SPC32H rep-HA ant-HAThis study
    Escherichia coli
        BTH101F cya-99 araD139 galE15 galK16 rpsL1(Strr) hsdR2 mcrA1 mcrB1; reporter strain in bacterial two-hybrid assay29
        BL21(DE3)F ompT hsdS (rB mB) gal (DE3); protein overexpressionLaboratory collection
Bacteriophages
    SPC32HInfect S. Typhimurium; O antigen specificThis study
    SPC32NInfect S. Typhimurium; O antigen specificThis study
    SPC32H ΔantSPC32H derivative with ΔantThis study
    SPC32H m1SPC32H derivative with one nucleotide substitution in tsp gene (C to T)This study
    SPC32H m2SPC32H derivative with one nucleotide substitution in ant gene promoter (G to T)This study
    SPC32H m12SPC32H derivative with two nucleotide substitutions in tsp gene (C to T) and ant gene promoter (G to T)This study
  • a Strr, streptomycin resistant.