Table 3

Inhibition of enzyme activity by N1-specific MAbs

MAb groupMAbIC50 for the indicated virus (ng/ml)a
BR/07CA/09VN/04
A3A210.4>>
4G27.7>>
B1H525.9>1,670
2D968.1>2,160
2G631.2>1,410
3H1023.6>1,250
4E927.0>2,420
C1C785.7>>
3C233.2>>
D2B528.2>>
3H463.4>>
E1H814.7>>
4B1250.9>8,599
2D4196>>
F4C41,184>>
CD6b>44.0>
Negative controlc>>>
  • a IC50, median inhibition concentration measured by ELLA of the reassortant H6N1 viruses containing the NA of each indicated virus (see Materials and Methods). >, greater than 32,000 ng/ml.

  • b CD6 is a MAb raised against 09pdm H1N1 NA in our laboratory using a protocol similar to that for generating BR/07 NA MAbs.

  • c Negative control, mouse MAb against influenza A virus nucleoprotein (ViroStat, Portland, ME).