Table 2

Epitopes proposed for the AAV:Fab complexes and their footprint areas

AAV:Fab complexProposed epitopeSurface area (Å2)c
NameDescriptiona
AAV1:4E4VR-IV456-AQNK-4591,197
VR-V492-TKTDNNN-498 (3-fold)b
AAV1(AAV6):5H7dVR-VT494 + 496-NNNS-4991,360
VR-VIII582-VN-583 + 588-STDPATGD-595 + H597
AAV2:C37-BVR-V492-SADNNNS-4981,230
VR-VIII585-RGNRQ-589 (3-fold)b
AAV5:3C5
    Site AVR-I254-SVDGSNAN-2611,728
VR-III374-DN-375
VR-VR483, 485-SVSAFATT-492, R494, E496, 499-GAS-501
    Site BVR-IQ2461,137
VR-VIIN530, 532-QPANPGT-538
HI loopS651, 653-VP-654, 656-SS-657 (5-fold)b
VR-IX704-DSTGE-708
  • a Residue numbering corresponds to that for each of the individual serotypes.

  • b Symmetry relation of residues to the reference VP monomer.

  • c Surface area calculation taken from PDBePISA (84) and rounded to the nearest Å2.

  • d AAV1(AAV6), 5H7 cross-reacts with AAVI and AAV6. Capsid residues 582-VN-583 and H597 near VR-VIII and T494 near VR-V flank (+) the indicated sites but do not interact directly with the CDRs in the docked Fab models.