Table 8

Loss-of-binding residues identified by prM-E protein display on HEK 293T cells

MAbDomainAmino acid residue(s)aEpitopeb
DV4-E4DIIR99, G102, G106, L107Fusion loop
DV4-E29DIIIY377F-strand
H390G-strand
DV4-E33DIIID375, Y377F-strand
H390, F392G-strand
DV4-E40DIIIK310A-strand
D375, Y377F-strand
H390, F392G-strand
DV4-E75DIIIK325B-strand
N360, T361DE loop
DV4-E76DIIIM301N-terminal linker
E338, V347, V348B-strand
R350CC′ loop
P356D-strand
L387G-strand
DV4-E78DIIIE338, V347, R350CC′ loop
DV4-E87DIIIM301N-terminal linker
E338, V347CC′ loop
P356D-strand
DV4-E88DIIIA331BC loop
T361DE loop
DV4-E121DIIIK325B-strand
A331BC loop
T361DE loop
  • a Amino acids were determined based on loss of binding to the indicated MAb by using prM-E display of a shotgun alanine-scanning mutagenesis library (see Materials and Methods for details).

  • b Loops and strands that comprised specific epitopes were assigned based on existing crystal structures of DIII from DENV-1, DENV-2, and DENV-3 E proteins (17, 19, 20, 89).