Table 7

Neutralization of DENV-4 strains 1036 (genotype I) and P75-514 (sylvatic genotype) based on a panel of DENV-4 MAbsa

VirusMAbTemp (°C)% neutralization of supernatant at dilution of:
Neat1:51:25
1036DV4-E337945829
1036DV4-E3401009872
P75-514DV4-E337999163
P75-514DV4-E340999986
1036DV4-E43784231
1036DV4-E440998333
P75-514DV4-E437946935
P75-514DV4-E440979278
1036DV4-E273768246
1036DV4-E2740823812
P75-514DV4-E2737895329
P75-514DV4-E2740989270
1036DV4-E2937693615
1036DV4-E2940957850
P75-514DV4-E2937603120
P75-514DV4-E2940785951
1036DV4-E3337907032
1036DV4-E3340999265
P75-514DV4-E3337999080
P75-514DV4-E334010010097
1036DV4-E4037794023
1036DV4-E4040987850
P75-514DV4-E4037957958
P75-514DV4-E4040999889
1036DV4-E6837633216
1036DV4-E6840887029
P75-514DV4-E6837793911
P75-514DV4-E6840958247
1036DV4-E72371600
1036DV4-E72402140
P75-514DV4-E723743711
P75-514DV4-E7240847470
1036DV4-E7637914923
1036DV4-E76401009661
P75-514DV4-E7637956430
P75-514DV4-E7640978468
1036DV4-E783779326
1036DV4-E7840977634
P75-514DV4-E783785335
P75-514DV4-E784091606
1036DV4-E873768279
1036DV4-E8740905624
P75-514DV4-E8737875017
P75-514DV4-E8740907332
1036DV4-E121371009357
1036DV4-E121401009664
P75-514DV4-E1213745416
P75-514DV4-E12140241431
1036DV4-E138372873
1036DV4-E138403189
P75-514DV4-E1383752185
P75-514DV4-E13840532825
  • a Hybridoma supernatant (neat or diluted) was incubated with 102 FFU of DENV-4 (strain 1036 or P75-514) for 1 h at 37°C or 40°C. Virus-MAb mixtures were added to Vero cell monolayers for 1 h at 37°C prior to addition of a methylcellulose overlay. DENV-infected foci were stained with a human-mouse chimeric E18 MAb. The percent neutralization was determined compared to medium alone. Results are representative of three independent experiments.