Table 5

Neutralization of other DENV-4 strains by cross-reactive fusion loop MAbs

VirusaGenotypebMAbTemp (°C)Time (h)EC50 (ng/ml)EC90 (ng/ml)
H241IE60371>5,000>5,000
H241IE603731,171>5,000
H241IE6040165711
H241IE86371>5,000>5,000
H241IE863732,534>5,000
H241IE8640195700
H241IE1063714,500>5,000
H241IE1063731,472>5,000
H241IE1064011831,750
H241IE119371>5,000>5,000
H241IE119373>5,000>5,000
H241IE119401267>5,000
TVP-376IIE603711,054>5,000
TVP-376IIE603731533,524
TVP-376IIE6040110113
TVP-376IIE86371594>5,000
TVP-376IIE86373952,104
TVP-376IIE864019285
TVP-376IIE106371510>5,000
TVP-376IIE1063731212,864
TVP-376IIE1064019166
TVP-376IIE1193711,165>5,000
TVP-376IIE119373213>5,000
TVP-376IIE119401105640
TVP-986IIE60371900>5,000
TVP-986IIE6037356548
TVP-986IIE6040111282
TVP-986IIE86371756>5,000
TVP-986IIE8637322832
TVP-986IIE8640131644
TVP-986IIE106371458>5,000
TVP-986IIE10637351809
TVP-986IIE10640122411
TVP-986IIE119371546>5,000
TVP-986IIE119373602,259
TVP-986IIE11940137621
P75-514SE603712682,317
P75-514SE6037345313
P75-514SE6040138454
P75-514SE86371981,758
P75-514SE8637325214
P75-514SE8640158306
P75-514SE1063711361,312
P75-514SE10637330285
P75-514SE10640133210
P75-514SE119371991,507
P75-514SE11937323380
P75-514SE1194017115
  • a Increasing concentrations (5 to 5,000 ng/ml) of the indicated MAbs were premixed with 102 FFU of DENV-4 (strain H241, TVP-376, TVP-986, or P75-514) for 1 h at 37°C, 3 h at 37°C, or 1 h at 40°C. Virus-MAb mixtures were added to Vero cell monolayers for 1 h at 37°C prior to addition of a methylcellulose overlay. DENV-4-infected foci were stained with a human-mouse chimeric E18 MAb. EC50 and EC90 values were determined by using nonlinear regression models (GraphPad Prism). The results are the averages of three independent experiments performed in triplicate.

  • b Summary of genotypes: H-241 is genotype I; 1036, TVP-376, and TVP-986 are genotype II; P75-514 is the sylvatic genotype (S).