Table 4

Neutralization of DENV-4 strain 1036 by cross-reactive fusion loop- and type-specific MAbsa

MAbTemp (°C)Time (h)EC50 (ng/ml)EC90 (ng/ml)
E603712,786>5,000
E60373833>5,000
E6040134195
E86371822>5,000
E86373232>5,000
E8640123125
E1063711,141>5,000
E106373271>5,000
E10640135178
E1193711,730>5,000
E119373598>5,000
E11940173442
DV4-E7537163258
DV4-E7537336181
DV4-E7540136103
DV4-E883711849
DV4-E88373934
DV4-E884011751
  • a Increasing concentrations (5 to 5,000 ng/ml) of the indicated MAbs were premixed with 102 FFU of DENV-4 (strain 1036) for 1 h at 37°C, 3 h at 37°C, or 1 h at 40°C. Virus-MAb mixtures were added to Vero cell monolayers for 1 h at 37°C prior to addition of a methylcellulose overlay. DENV-infected foci were stained with a human-mouse chimeric E18 MAb. EC50 and EC90 values were determined by using nonlinear regression models (GraphPad Prism). The results are the averages of three independent experiments performed in triplicate.