Table 3

Neutralization of DENV-1, DENV-2, and DENV-3 by cross-reactive fusion loop-specific MAbsa

MAbIsotypeDENV-1DENV-2DENV-3
EC50 (ng/ml)EC90 (ng/ml)EC50 (ng/ml)EC90 (ng/ml)EC50 (ng/ml)EC90 (ng/ml)
E60IgG2a21661119537612>5,000
E86IgG2a83450343432642,089
E106IgG2a473091527584>5,000
E119IgG2a59406174911302,500
  • a Increasing concentrations (5 to 5,000 ng/ml) of the indicated MAbs were premixed with 102 FFU of DENV-1 (strain 16007), DENV-2 (strain 16681), or DENV-3 (strain 16652) for 1 h at 37°C. Virus-MAb mixtures were added to Vero cell monolayers for 1 h at 37°C prior to addition of a methylcellulose overlay. DENV-infected foci were stained with a human-mouse chimeric E18 MAb. EC50 and EC90 values were determined by using nonlinear regression models (GraphPad Prism). The results are the averages of three independent experiments performed in triplicate.