Table 1

Features of predicted protein-coding regions in EEHV1A and EEHV1B

EEHV1 ORFHCMV orthologIdentitya (%)ORF familyProtein namebProtein description
EE195Protein EE1
EE299Protein EE2
EE332EE3Protein EE3Contains signal peptide
EE442EE3Protein EE4Contains signal peptide
EE551Protein EE5Contains signal peptide
U82UL11570Envelope glycoprotein LContains signal peptide; complexed with envelope glycoprotein H; involved in cell entry; involved in cell-to-cell spread
U81UL11473Uracil-DNA glycosylaseInvolved in DNA repair
EE6cProtein EE6Contains signal peptide
U79UL11289Protein UL112Involved in gene regulation; involved in DNA replication
U77UL105100Helicase-primase helicase subunitInvolved in DNA replication
U76UL104100Capsid portal proteinDodecamer located at one capsid vertex in place of penton; involved in DNA encapsidation
U75UL10398Tegument protein UL7*Involved in virion morphogenesis
U74UL10298Helicase-primase subunitInvolved in DNA replication
U7398DNA replication origin-binding helicaseInvolved in DNA replication
U72UL10097Envelope glycoprotein MType 3 membrane protein; 8 transmembrane domains; complexed with envelope glycoprotein N; involved in virion morphogenesis; involved in membrane fusion
U71UL9991Myristylated tegument proteinEnvelope-associated; involved in virion morphogenesis
U70UL9898DNaseInvolved in DNA processing
U69UL97100Tegument serine/threonine protein kinaseInvolved in protein phosphorylation
U68UL96100Tegument protein UL14*Involved in virion morphogenesis
U67UL95100Protein UL95Promotes accumulation of late transcripts
U65UL9496Tegument protein UL16*Possibly involved in virion morphogenesis
U64UL9399DNA packaging tegument protein UL17*Capsid-associated; involved in DNA encapsidation; involved in capsid transport
U63UL9299Protein UL92
U62UL9199Protein UL91
U60UL8999DNA packaging terminase subunit 1Contains ATPase domain; involved in DNA encapsidation
U59UL88100Tegument protein UL88
U58UL87100Protein UL87Promotes accumulation of late transcripts
U57UL8699Major capsid protein6 copies form hexons, 5 copies form pentons; involved in capsid morphogenesis
U56UL8599Capsid triplex subunit 2Complexed 2:1 with capsid triplex subunit 1 to connect capsid hexons and pentons; involved in capsid morphogenesis
U54UL82/UL83/UL8498DURPProtein U54
U53.5UL80.5100Capsid scaffold proteinClipped near C terminus; involved in capsid morphogenesis
U53UL80100Capsid maturation proteaseSerine protease (N-terminal region); minor scaffold protein (remainder of protein, clipped near C terminus); involved in capsid morphogenesis
U52UL79100Protein UL79Promotes accumulation of late transcripts
U51UL7898Envelope protein UL78Type 3 membrane protein; 7 transmembrane domains; similar to GPCRs; putative chemokine receptor; possibly involved in intracellular signaling
U50UL7798DNA packaging tegument protein UL25*Located on capsid near vertices; possibly stabilizes the capsid and retains the genome; involved in DNA encapsidation
U49UL76100Nuclear protein UL24*
EE782Thymidine kinaseInvolved in nucleotide metabolism
U48UL7566Envelope glycoprotein HType 1 membrane protein; possible membrane fusogen; complexed with envelope glycoprotein L; involved in cell entry; involved in cell-to-cell spread
U47UL7463Envelope glycoprotein OAssociated with envelope glycoprotein H and envelope glycoprotein L; involved in virion morphogenesis
U46UL7382Envelope glycoprotein NType 1 membrane protein; complexed with envelope glycoprotein M; involved in virion morphogenesis; involved in membrane fusion
EE874Protein EE8Contains potential transmembrane domain
U27UL4499DNA polymerase processivity subunitdsDNA-binding protein; involved in DNA replication
EE9100Ribonucleotide reductase subunit 2Involved in nucleotide metabolism
U28UL45100Ribonucleotide reductase subunit 1Involved in nucleotide metabolism
U29UL4699Capsid triplex subunit 1Complexed 1:2 with capsid triplex subunit 2 to connect capsid hexons and pentons; involved in capsid morphogenesis
U30UL4798Tegument protein UL37*Complexed with large tegument protein; involved in virion morphogenesis
U31UL4896Large tegument proteinComplexed with tegument protein UL37; ubiquitin-specific protease (N-terminal region); involved in capsid transport
U32UL48A89Small capsid proteinLocated externally on capsid hexons; involved in capsid morphogenesis; possibly involved in capsid transport
U33UL4999Protein UL49
U34UL50100Nuclear egress membrane proteinType 2 membrane protein; interacts with nuclear egress lamina protein; involved in nuclear egress
U35UL51100DNA packaging protein UL33*Interacts with DNA packaging terminase subunit 2; involved in DNA encapsidation
U36UL52100DNA packaging protein UL32*Involved in DNA encapsidation; possibly involved in capsid transport
U37UL53100Nuclear egress lamina proteinInteracts with nuclear egress membrane protein; involved in nuclear egress
U38UL5497DNA polymerase catalytic subunitInvolved in DNA replication
U39UL5587Envelope glycoprotein BType 1 membrane protein; possible membrane fusogen; binds cell surface heparan sulfate; involved in cell entry; involved in cell-to-cell spread
U40UL56100DNA packaging terminase subunit 2Involved in DNA encapsidation
U41UL57100Single-stranded DNA-binding proteinContains zinc finger; involved in DNA replication; possibly involved in gene regulation
U42UL69100Multifunctional expression regulatorRNA-binding protein; shuttles between nucleus and cytoplasm; inhibits pre-mRNA splicing; exports virus mRNA from nucleus; exerts most effects posttranscriptionally; involved in gene regulation; involved in RNA metabolism and transport
U43UL7099Helicase-primase primase subunitInvolved in DNA replication
U44UL71100Tegument protein UL51*Involved in virion morphogenesis
EE1099Protein EE10
EE11100UL27Protein EE11
U4UL27100UL27Protein UL27
U11UL3299Tegument protein pp150Major tegument protein; binds to capsids
U12UL33100Envelope glycoprotein UL33Type 3 membrane protein; 7 transmembrane domains; similar to GPCRs; putative chemokine receptor; involved in intracellular signaling
UL34UL34100Protein UL34Involved in gene regulation
U14UL3595Tegument protein UL35
EE1294Protein EE12Contains potential transmembrane domain
EE13100Protein EE13Contains US22 domain
EE14100Protein EE14
EE1590Protein EE15Contains potential transmembrane domain
EE1662Protein EE16Contains signal peptide
EE1772Protein EE17Contains signal peptide
EE18997TMMembrane protein EE18Type 3 membrane protein; 7 transmembrane domains
EE19997TMMembrane protein EE19Type 3 membrane protein; 7 transmembrane domains
EE20100EE20Membrane protein EE20Type 2 membrane protein
EE21997TMMembrane protein EE21Type 3 membrane protein; 7 transmembrane domains; similar to GPCRs
EE2278OX-2Membrane protein EE22Type 2 membrane protein; contains 2 immunoglobulin domains; similar to OX-2 (CD200)
EE23100OX-2Protein EE23Contains signal peptide; contains immunoglobulin domain; similar to OX-2 (CD200)
EE24100Protein EE24Contains potential transmembrane domain
EE2599Protein EE25Contains potential transmembrane domain
EE26947TMMembrane protein EE26Type 3 membrane protein; 7 transmembrane domains; similar to GPCRs
EE27100Protein EE27
EE281007TMMembrane protein EE28Type 3 membrane protein; 7 transmembrane domains; similar to GPCRs
EE29100DURPProtein EE29
EE3098Protein EE30
EE31967TMMembrane protein EE31Type 3 membrane protein; 7 transmembrane domains
EE3290EE20Protein EE32Contains potential transmembrane domain
EE331007TMMembrane protein EE33Type 3 membrane protein; 7 transmembrane domains
EE34997TMMembrane protein EE34Type 3 membrane protein; 7 transmembrane domains; similar to GPCRs
EE351007TMMembrane protein EE35Type 3 membrane protein; 6 transmembrane domains
EE36997TMMembrane protein EE36Type 3 membrane protein; 7 transmembrane domains
EE371007TMMembrane protein EE37Type 3 membrane protein; 7 transmembrane domains
EE381007TMMembrane protein EE38Type 3 membrane protein; 7 transmembrane domains
EE39997TMMembrane protein EE39Type 3 membrane protein; 7 transmembrane domains
EE40e97g7TMMembrane protein EE40Type 3 membrane protein; 7 transmembrane domains
EE41997TMMembrane protein EE41Type 3 membrane protein; 7 transmembrane domains
EE421007TMMembrane protein EE42Type 3 membrane protein; 7 transmembrane domains
EE431007TMMembrane protein EE43Type 3 membrane protein; 7 transmembrane domains
EE44e,f75gProtein EE44Contains signal peptide
EE45937TMMembrane protein EE45Type 3 membrane protein; 8 transmembrane domains; similar to GPCRs
EE4699β-1,3-Galactosyl-O-glycosyl-glycoprotein β-1,6-N-acetylglucosaminyltransferaseContains potential transmembrane domain; involved in protein glycosylation
EE47957TMMembrane protein EE47Type 3 membrane protein; 8 transmembrane domains; similar to GPCRs
EE48997TMMembrane protein EE48Type 3 membrane protein; 7 transmembrane domains
EE49967TMMembrane protein EE49Type 3 membrane protein; 8 transmembrane domains
EE50100EE50Membrane protein EE50Contains signal peptide; contains immunoglobulin domain
EE5197OX-2Membrane protein EE51Type 2 membrane protein; contains 2 immunoglobulin domains; similar to OX-2 (CD200)
EE5288EE50Membrane protein EE52Type 2 membrane protein; contains immunoglobulin domain
EE53f45gEE50Membrane protein EE53Type 2 membrane protein; contains immunoglobulin domain
EE54dEE50Membrane protein EE54Type 2 membrane protein
EE55d,eEE50Membrane protein EE55Type 2 membrane protein
EE56dEE50Membrane protein EE56Type 2 membrane protein
EE57e,f82gEE50Membrane protein EE57Type 2 membrane protein; contains immunoglobulin domain
EE58e51gEE50Membrane protein EE58Type 2 membrane protein
EE59f,h7TMMembrane protein EE59Type 3 membrane protein; 7 transmembrane domains
EE60c,fEE50Membrane protein EE60Type 2 membrane protein; contains immunoglobulin domain
EE61c,fEE50Membrane protein EE61Type 2 membrane protein
EE62c7TMMembrane protein EE62Type 3 membrane protein; 7 transmembrane domains
EE6358α-(1,3)-FucosyltransferaseContains potential transmembrane domain; involved in protein glycosylation
  • a Calculated from EEHV1A and EEHV1B amino acid sequences aligned by using GCG Gap and shown to the nearest integer.

  • b The standard nomenclature for orthologous herpesvirus proteins employed in NCBI Reference Sequence files is used for conserved ORFs. In this system, the names of proteins specified by certain ORFs are derived from HCMV nomenclature (e.g., tegument protein UL35, encoded by U14). However, the names of proteins specified by certain core ORFs are derived from nomenclature of the alphaherpesvirus herpes simplex virus type 1 (e.g., DNA packaging tegument protein UL25, encoded by U50). Names in the latter category may cause confusion in relation to HCMV ORF nomenclature and are marked by asterisks.

  • c ORF absent from EEHV1B.

  • d ORF absent from EEHV1A.

  • e Fragmented in EEHV1B.

  • f Fragmented in EEHV1A.

  • g Repaired, nonfragmented sequences used.

  • h EEHV1A ORF too fragmented to be repaired.