Table 1

Properties of empty or full CPV capsid entry into CRFK or TRVb cellsa

ParameterValue for expt (nb)
CRFK cellsExpt 4, TRVbc cells (empty capsids)
Expt 1 (empty capsids)Expt 2 (full capsids)Expt 3
Empty capsidsFull capsids
% entry (no. of total CPV attachments; no. of cells analyzed)24 (628; no. of cells analyzed 4 cells)25 (121; no. of cells analyzed 6 cells)35 (54; no. of cells analyzed 3 cells)25 (68; no. of cells analyzed 3 cells)27 (118; no. of cells analyzed 4 cells)
Capture mode as % of entry events (no. of total entry events)80% diffuse into pit; 9% land in/near pit; 11% stop diffusing before pit detection (347)80% diffuse into pit; 0% land in/near pit; 20% stop diffusing before pit detection (30)88% diffuse into pit; 0% land in/near pit; 12% stop diffusing before pit detection (19)100% diffuse into pit; 0% land in/near pit; 0% stop diffusing before pit detection (17)95% diffuse into pit; 2% land in/near pit; 3% stop diffusing before pit detection (255)
Avg diffusion coefficient0.014 μm2 s−1 (101)NTNTNTNT
Time (avg no. of s ± SD) between CPV attachment and capture15 ± 14 (145)44 ± 47 (30)*67 ± 58 (22)*26 ± 26 (17)*#23 ± 19 (51)*
Time (avg no. of s ± SD) between pit detection and CPV capture13 ± 13 (309)13 ± 14 (30)13 ± 11 (20)15 ± 16 (17)10 ± 10 (231)*
Time between pit detection and CPV capture as % total pit lifetime25 ± 20 (309)19 ± 20 (30)20 ± 19 (20)21 ± 19 (17)25 ± 21 (231)
Total lifetime (avg no. of s ± SD) of CPV pits53 ± 19 (339)65 ± 19 (30)*60 ± 18 (20)*63 ± 27 (17)*40 ± 13 (231)*
Mean residence time (avg no. of s ± SD) of capsids that did not enter39 ± 44 (463)62 ± 55 (91)*71 ± 55 (35)*60 ± 46 (51)*46 ± 27 (71)
  • a *, statistical significance (P < 0.05, Student's t test) relative to corresponding data from experiment 1; #, statistical significance (P < 0.05, Student's t test) relative to corresponding data from empty capsids in experiments 1 and 3; NT, not tested.

  • b n, number of events analyzed except where otherwise noted under “Parameters.”

  • c TRVb cells expressing fTfR-eGFP.