Table 1

Analysis of gene expression by qRT-PCR

GeneRelative fold induction (avg ± SD) for the indicated treatment and dpca
CombinationAd5-boIFN-αAd5-boIFN-λ3PBS
012012012012
IFN-β1.5 ± 0.71.5 ± 0.61.3 ± 0.61.5 ± 0.51.3 ± 0.61.7 ± 0.61.8 ± 0.51.2 ± 0.21.7 ± 0.41.6 ± 0.42.1 ± 0.11.1 ± 0.1
IFN-λ31.4 ± 0.81.4 ± 0.61.2 ± 0.71.1 ± 0.91.3 ± 0.91.8 ± 1.10.4 ± 0.10.4 ± 0.11.6 ± 2.23.1 ± 1.69.6 ± 2.03.6 ± 0.8
IL-28 Rα1.0 ± 0.31.0 ± 0.41.0 ± 0.61.4 ± 0.51.6 ± 0.51.7 ± 0.11.0 ± 0.10.7 ± 0.01.7 ± 0.91.6 ± 0.42.5 ± 0.61.7 ± 0.4
IL-10 Rβ1.6 ± 0.31.1 ± 0.31.1 ± 0.21.0 ± 0.01.4 ± 0.21.9 ± 0.21.4 ± 0.21.3 ± 0.31.7 ± 0.31.2 ± 0.12.2 ± 0.11.9 ± 0.2
CCL25.3 ± 4.44.9 ± 5.21.3 ± 1.01.9 ± 1.71.9 ± 1.22.2 ± 1.44.0 ± 0.91.4 ± 0.34.2 ± 0.91.5 ± 0.58.4 ± 6.710.8 ± 3.2
CCL30.5 ± 0.10.4 ± 0.20.5 ± 0.30.5 ± 0.10.4 ± 0.10.6 ± 0.10.7 ± 0.30.8 ± 0.31.1 ± 0.71.2 ± 0.41.2 ± 0.21.5 ± 0.3
CCL201.0 ± 0.31.0 ± 0.11.1 ± 0.31.1 ± 0.11.4 ± 0.61.6 ± 1.21.3 ± 0. 51.4 ± 0.82.0 ± 1.32.2 ± 0.83.9 ± 1.01.8 ± 0.9
CXCL1014.9 ± 10.79.3 ± 3.311.2 ± 12.110.0 ± 2.26.7 ± 2.016.1 ± 9.555.2 ± 52.07.1 ± 2.410.0 ± 3.21.4 ± 0.124.1 ± 23.731.9 ± 5.2
IRF77.2 ± 4.32.6 ± 1.02.7 ± 1.34.0 ± 1.15.3 ± 5.06.1 ± 4.511.3 ± 11 0.41.3 ± 0.42.4 ± 1.81.2 ± 0.710.5 ± 9.75.1 ± 3.0
ISG1555.6 ± 31.612.3 ± 3.316.5 ± 2.022.7 ± 11.610.2 ± 4.821.4 ± 1.140.8 ± 18.67.0 ± 2.110.0 ± 2.40.5 ± 0.390.0 ± 13325.9 ± 16.5
MDA56.6 ± 0.85.4 ± 2.14.6 ± 2.23.7 ± 0.72.4 ± 0.94.6 ± 1.67.0 ± 1.12.8 ± 0.83.5 ± 1.01.3 ± 0.38.6 ± 7.77.9 ± 0.9
MX19.2 ± 1.95.4 ± 3.17.0 ± 4.59.8 ± 1.56.9 ± 2.113.1 ± 3.65.8 ± 1.55.4 ± 1.36.3 ± 1.61.5 ± 0.317.1 ± 14.714.2 ± 1.0
OAS112.6 ± 3.110.0 ± 2.09.1 ± 2.914.9 ± 3.49.1 ± 3.115.3 ± 4.422.9 ± 10.47.9 ± 1.610.7 ± 4.31.7 ± 0.826.0 ± 30.315.6 ± 8.4
PKR6.4 ± 0.84.4 ± 1.93.8 ± 2.36.7 ± 1.24.9 ± 2.08.3 ± 2.97.9 ± 2.33.3 ± 0.54.0 ± 1.20.8 ± 0.19.3 ± 8.46.8 ± 0.9
RIGI17.0 ± 1.611.4 ± 4.79.6 ± 5.013.4 ± 3.310.3 ± 4.714.5 ± 6.927.4 ± 15.18.8 ± 0.914.0 ± 3.71.9 ± 0.527.0 ± 31.120.6 ± 4.7
  • a RNA was extracted from PBMCs of the animals from experiment II at 0, 1, and 2 days postchallenge with FMDV by aerosol (equivalent to −1, 0, and 1 days postinoculation with PBS or Ad5s). Gene expression was measured by RT-PCR and expressed as the group average relative fold induction compared to the values before treatment (−1 dpi). Shading denotes >2-fold induction.