Table 1

Expression of the chimeras was tested by flow cytometry with MAb F1a and an F-specific pAba

Construct% of F2 at 150 min of chase (%WT)Amt (mean ± SD) of F on the cell surface (%WT)
F1apAb
PIV5 F100.0100.0100.0
P-Me-Me-Me68.537.9 ± 4.2**69.7 ± 3.3*
P-Mu-Mu-Mu22.023.8 ± 5.1**28.6 ± 6.5*
P-N-N-N73.691.3 ± 8.5*79.2 ± 4.5*
P-h-h-h107.588.2 ± 6.6*81.3 ± 2.9*
P-Ni-Ni-Ni76.922.9 ± 3.8*41.2 ± 3.9*
P-Me-Me-P117.837.7 ± 3.4*98.9 ± 7.5*
P-Mu-Mu-P30.723.9 ± 8.2**32.7 ± 4.5*
P-N-N-P58.840.1 ± 5.3***57.1 ± 3.0*
P-h-h-P62.950.9 ± 7.8**61.8 ± 5.8*
P-Ni-Ni-P19.414.9 ± 6.3**17.7 ± 5.2*
P-P-Me-Me32.631.1 ± 4.8**96.7 ± 7.9*
P-P-Mu-Mu20.436.4 ± 9.2*42.9 ± 2.8*
P-P-N-N93.078.0 ± 3.9*90.4 ± 0.6*
P-P-h-h92.996.7 ± 4.9*99.3 ± 2.2*
P-P-Ni-Ni46.924.6 ± 0.1***49.4 ± 4.8*
P-P-P-Me107.656.4 ± 7.3*55.6 ± 4.1*
P-P-P-Mu65.837.9 ± 2.8**41.4 ± 6.5*
P-P-P-N173.1102.0 ± 7.3*115.1 ± 3.5*
P-P-P-h103.699.0 ± 4.5*99.4 ± 1.4*
P-P-P-Ni35.925.8 ± 0.5***40.8 ± 0.9*
P-P-Me-P28.025.6 ± 6.3**60.4 ± 4.7*
P-P-Mu-P23.938.9 ± 12.1*42.3 ± 0.9*
P-P-N-P65.762.3 ± 5.3*64.0 ± 8.7*
P-P-h-P90.793.4 ± 4.0*100.9 ± 0.3*
P-P-Ni-P74.976.8 ± 9.9*94.4 ± 3.0*
P-Me-P-P27.542.3 ± 3.9**64.4 ± 4.6*
P-Mu-P-P75.195.7 ± 11.2*91.1 ± 1.1*
P-N-P-P81.4105.1 ± 11.2*80.6 ± 4.9*
P-h-P-P78.261.8 ± 5.6*57.0 ± 9.6*
P-Ni-P-P30.043.2 ± 3.8**52.5 ± 8.9*
  • a As determined by flow cytometry (n = 3).

  • * , P < 0.05;

  • ** , P < 0.01;

  • *** , P < 0.001. The extent of cleavage was determined by 35S radiolabel, pulse-chase immunoprecipitation using an F-specific polyclonal antibody (pAb) and SDS-PAGE. The amount of F2 at 150 min after chase is indicated as the percentage of the wild type (%WT).