Table 3

Envelope coreceptor use and relationship to neutralization phenotype

Vector and subjectEnvelopeInfectivity on NP2 cells with use of the following coreceptor:ClassificationaNeutralization phenotypeb
CD4/CCR5CD4/CXCR4CD4 aloneCCR5 aloneCXCR4 alone
7312A-SNAGc
    TD02CA4258.1181.5NTd0.1NTR5S
    TD05CA370.1620.4NTNTR5S
    TD06CA4401.153NTNTR5S
    TD08CA381.4e2550.11.2NTR5S
    TD09CA4145.8627NT0.8NTR5S
    TD11CA4272.163300.21.5NTR5S
CA65331.311050.00.8NTR5/X4S
    TD12CA65332.142.5NT0.1NTR5S
    TD13CA4153.92,5002.01.30.9NTR5S
    TD15CA4137.21,328NTNTR5S
CA4137.197,1251.41.71.2NTR5R
    TD16CA4182.4273NTNTR5R
CA4182.124,6871.00.91.1NTR5S
CA65410.32250.20.8NTR5S
CA65410.5134.50.11.50.5NTR5S
CA65410.73000.42.50.9NTR5S
CA65410.1359.5NTNTR5S
    TD17CA4205.18502.52.11.1NTR5R
CA4205.81,1005.53.01.4NTR5R
    TD19CA4269.21,7817.56.30.2NTR5S
CA4269.62,625NT1.1NTR5R
CA65339.332NTNTR5R
    TD20CA4206.725NTNTNTR5S
    TD21CA4013.53,1872.41.90.9NTR5R
CA4013.822.5NTNTNTR5S
    NAfNL4.3212.5NTNTNTX4NA
pcDNA3.1V5-His-TOPOg
    NAROD A69,87731,250250112112R5/X4R
    NAhROD B1,25031,25050<5013,975X4/CD4 independentS
    TD01CA330.569,877559559250112R5S
    TD08CA381.4e69,877250112250112R5S
    TD14CA316.9349,3861,250559559559R5S
    TD07CA319.7349,3862,795559559559R5S
    TD18CA409.14156,2501,2501,2501,2501,250R5R
    NACA7205.8349,3862,7951121,869250R5S
  • a Viruses were classified as dual tropic if the titer on the alternative coreceptor was within 1.5 log10 units of the titer obtained from the dominant coreceptor cell line (4).

  • b S, sensitive; R, resistant.

  • c HIV-2 gp140 cloned into full-length HIV-2 7312A-SNAG vector. NL4.3 refers to virus derived from the full-length HIV-1 NL4.3 molecular clone. For infectivity on NP2 cells with the 7312A-SNAG vector, units are 103 FFU/ml, where FFU represents the number of focus-forming units measured by immunostaining of NP2 cells with or without CD4 and with or without coreceptors following infection with live virus.

  • d NT, not tested.

  • e Coreceptor tropism tested in both formats.

  • f NA, subject identifier not assigned as envelope not used for autologous neutralization studies.

  • g HIV-2 gp160 cloned into expression vector pcDNA3.1V5-His-TOPO. For infectivity on NP2 cells with the pcDNA3.1V5-His-TOPO vector, units are TCID50s, where TCID50s were calculated according to luciferase readout in NP2 cells following infection with pseudovirus produced by cotransfection of HIV-2 gp160 envelope plasmid and NL4.3LucRE.

  • h Bold indicates ROD B, the only CD4-independent isolate of those tested.