Table 2

Antibody titers in humanized mice

Mouse IDEngraftment (%)Donor IDTreatmentDay collectedAnti-DENV titeraTotal IgM (ng/ml)DENV IgM (ng/ml)Total IgG (ng/ml)bDENV IgG (ng/ml)Homologous neut50c titer
638624725 mosq.e bites30Neg26,169653
699517374 mosq. bites301:16015,3706,436NegNeg
701587374 mosq. bites301:2073,8406,23313,478Neg<10
713207895 mosq. bites30Neg5,0311,864
714777895 mosq. bites30Neg1,171191
715207894 mosq. bites301:640118,20066,81116,529Neg<10
664594915 mosq. bites41:2560119,363134,21857,500135>10
665474915 mosq. bites41:64025,23030,1612,686Neg>10
666304914 mosq. bites41:8046,941NDdNegND
702497374 mosq. bites12Neg9,4593,386
703247375 mosq. bites12Neg10,360NegNeg
704807374 mosq. bites121:2049,0972,068Neg
705497885 mosq. bites32Neg41,4171,387
706827885 mosq. bites321:4084,0906,605NegNeg
707737884 mosq. bites321:2069,8403,232NegNeg
643114724 mosq. bites32Neg18,400Neg
712737885 mosq. bites32Neg52,6003,125
682885344 mosq. bites321:160161,00018,26795,650Neg<10
716187894 mosq. bites32Neg145,00023,950
723542184 mosq. bites32Neg8,6009,650
725582184 mosq. bites32Neg71,200Neg
724402184 mosq. bites32NegNegNeg
729662184 mosq. bites321:2051,2004,1482,450Neg<10
722412185 mosq. bites32Neg23,90011,750
717227895 mosq. bites32Neg26,5004,531
59240353Inoculation30Neg
4 mosq. bites 210 days p.i.141:2560167,000141,4981,360Neg<10
61345261Inoculation30Neg
4 mosq. bites 210 days p.i.14Neg28,3001,090
63381471Inoculation + saliva30Neg
4 mosq. bites 182 days p.i.14Neg9,5001,950
63471471Inoculation + saliva30Neg
4 mosq. bites 182 days p.i.14Neg32,500Neg
66148291Inoculation + saliva30Neg
4 mosq. bites 182 days p.i.141:80150,8006,82293,000Neg<10
66259291Inoculation + saliva30Neg
4 mosq. bites 182 days p.i.14Neg59,7009,750
66343291Inoculation + saliva30Neg
4 mosq. bites 178 days p.i.141:320175,00021,68593,300Neg<10
74046316Uninfected, 4 bites4Neg164,480Neg
12Neg152,380Neg
32Neg211,111232
74151316Uninfected, 4 bites4Neg113,760128
12Neg111,520143
32Neg145,160304
74254316Uninfected, 4 bites4Neg153,36061,605
12Neg147,04067,536
32Neg201,834116,666
74342316Uninfected, 4 bites4Neg137,240293
12Neg131,160270
32Neg150,420548
74443316Uninfected, 4 bites4Neg110,3207,644
12Neg93,72012,725
32Neg88,04645,523
74544316Uninfected, 4 bites4Neg147,480Neg
12Neg124,000Neg
32Neg186,070Neg
Human serumPositive control>1:40,000686,36484,2545.5e61.5e5
  • a Neg, average optical density (OD) of negative controls plus 1 standard deviation (SD).

  • b Neg, <15.6 ng/ml for IgM and <7.8 ng/ml for IgG.

  • c neut50, 50% neutralization.

  • d ND, not done.

  • e mosq., mosquito.