Table 4

Specific HAI activities of human H2N2 antibodies against wild-type or mutated VLPs

MAbHAI activitya (μg/ml) against MAb H2N2 VLPs
WTG135DG135SR137QR137 MR137KK156ET193KT193AS228G
8F8<0.20.7<0.210>100.1><0.20.7
8M20.7>1.30.71.30.70.4100.72.5
2G1<0.20.7<0.2<0.2<0.2<0.2>1.3<0.20.4
  • a The highest titer that still showed HAI activity is shown. >, activity was not detected at the highest concentration tested, 20 μg/ml. VLPs are based on the HA of A/Japan/305+/1957 except for the K156 mutant, which is based on A/Singapore/1/1957. WT, wild type.