Table 2.

Gross differences in gene length (nt) and gene complement between Dryvax clonesa

GeneIDGene length (nt)
DPP clone no.:Dryvax
0910111213151617192021CL337372kDuke
TNF-α-receptor (CrmB) (Cop-C22L)_VAC_DVX_002441441441441441432441441441441441
TNF-α-receptor (CrmB) (Cop-C22L)_VAC_DVX_003285285285285285285285285285285285369369369369
Ankyrin-likeVAC_DVX_004336240240336336336336336336336336336342336336
Ankyrin-likeVAC_DVX_005387387387387387387387234387387387234387387234
Ankyrin-likeVAC_DVX_006414435435348414414435765435444339450450414441
Ankyrin (CPXV-008)_VAC_DVX_007453597597597453597453453597597453597597597597
Ankyrin (CPXV-008)_VAC_DVX_0081,1491,1491,1491,1491,1491,1491,1491,1491,1491,1491,1491,1491,1491,1491,149
Unknown (Cop-B22R)_VAC_DVX_009546546546546354546546546546546546546546546546
Unknown (Cop-C15L)_VAC_DVX_010264264264264264276270276270264270276276270276
Serpin (SPI-1) (Cop-C12L)_VAC_DVX_0131,0741,0741,0741,0741,0741,0741,0741,0741,0741,0741,0741,0051,0741,074930
EGF growth factor (Cop-C11R)_VAC_DVX_014423423423423423426426426426426426423420420426
Ubiquitin ligase VAC_DVX_016135135135135135135135135135135135135135135135
Ubiquitin ligase VAC_DVX_017252201201201252252252273201201201252252252252
Ubiquitin ligase VAC_DVX_018189189189189189189189189189189189189189189189
IL-18 BP (Bsh-D7L) (Cop-C12L)_VAC_DVX_019381381375375381381381381381381381375381375375
Ankyrin (Bang-D8L)_VAC_DVX_020273273273273273273273273273273273273273273273
Ankyrin (Bang-D8L)_VAC_DVX_021522522522522540522522522522429429429429
Ankyrin (Bang-D8L)_VAC_DVX_022513894513513513894408513498513513408408408408
Ankyrin (Bang-D8L)_VAC_DVX_023252252252252252252234252252252252234234234234
Ankyrin (Bang-D8L)_VAC_DVX_024216216216216216216216216216216216216216216216
Unknown (Tan-TC10L)_VAC_DVX_025168168168168168171171171168168168171171168168
Ankyrin (Cop-C9L)_VAC_DVX_026.1726726726726573
Ankyrin (Cop-C9L)_VAC_DVX_0261,9051,9051,9051,1911,1911,1911,1911,9051,9051,9058941,9051,9051,9051,905
Host range virulence factor (Cop-C7L)_VAC_DVX_028453453453453453453453453453453453453453453
Unknown (Cop-C5L)_VAC_DVX_030606606606606606606606606606606606615b618b615b606
IL-1 receptor antagonist (Cop-C4L)_VAC_DVX_032387387231231231231231387231387231411231411231
IL-1 receptor antagonist (Cop-C4L)_VAC_DVX_033180174180180180180180180180180180180180180
Putative TLRc signaling inhibitor (Cop-C1L)_VAC_DVX_036672675675675675675672675672675672675675675675
Ankyrin (Cop-M1L)_VAC_DVX_039219219219219219219219219219219
Ankyrin (Cop-M1L)_VAC_DVX_040645645645645855645645645
Ankyrin (Cop-M1L)_VAC_DVX_0414804831,2004861,2001,3834864834864861,1974801,4191,4101,410
Serpin (SPI-3) (Cop-K2L)_VAC_DVX_0441,1101,1101,1101,1101,1101,1101,1101,0951,1101,0951,0951,1101,1101,1101,110
Putative monoglyceride lipase (Cop-K5L)_VAC_DVX_047513516513513516516366366366516516366513411516
Putative monoglyceride lipase (Cop-K6L)_VAC_DVX_048246195195333246195333333246246195333246246246
Apoptosis inhibitor (Cop-F1L)_VAC_DVX_050681681669681681681681681681681681681681681681
Kelch-like, innate immune response modifier, virulence factor (Cop-F3L)_VAC_DVX_052.1678
Kelch-like (Cop-F3L)_VAC_DVX_0521,4341,4341,4341,4431,4341,4341,4346781,4431,4431,4341,4431,4341,4431,443
RhoA signaling inhibitor, virus release protein (Cop-F11L)_VAC_DVX_0601,0471,0471,0471,0471,0471,0471,0471,0471,0651,0471,0471,0471,0471,0471,047
Virosome component (Cop-E5R)_VAC_DVX_071570570996570507570570570570570570570570570996
Virosome component (Cop-E5R)_VAC_DVX_072450450450450450450450450450450450450450
DNA polymerase (Cop-E9L)_VAC_DVX_0763,0183,0213,0213,0213,0183,0183,0213,0183,0213,0183,0183,0183,0183,0183,018
Glutaredoxin 1 (Cop-O2L)_VAC_DVX_080324324324324324324324324324327324327327327327
Ribonucleotide red. large sub.d (Cop-I4L) _VAC_DVX_0842,3162,2922,3162,3102,3162,3162,3161,2512,2922,3162,3162,3162,3162,3162,316
Ribonucleotide red. large sub. VAC_DVX_084.11,152
IMV heparin binding surface protein (Cop-H3L) _VAC_DVX_112975957957975957975975957957975957957957975975
Core protein (Cop-A4L)_VAC_DVX_134840840822846846840840840846840840846846846846
Membrane protein, early morph (Cop-A9L)_VAC_DVX_139285327327327327327327327327327327327327327327
Structural protein (Cop-A12L)_VAC_DVX_142576576576576570576570576576576576576576579576
Virion maturation; IMV membrane protein (Cop-A13L)_VAC_DVX_143213213213213207207213213213213213213213213207
Myristylated entry/cell fusion protein (Cop-A16L) _VAC_DVX_1471,1371,1371,1371,1371,1341,1371,1371,1371,1371,1371,1371,1371,1341,1371,134
Holliday junction resolvase (Cop-A22R)_VAC_DVX_153531531531531531531531531531531531531564b564b531
Unknown (Cop-A37R)_VAC_DVX_171801801792801801801801792792792792792792792792
Semaphorin (Cop-A39R)_VAC_DVX_174681768399399399774687405681768681405681399687
Semaphorin (Cop-A39R)_VAC_DVX_175429429429429429429429429429207429429429
Lectin homolog (Cop-A40R)_VAC_DVX_176507507507507492507507507507507492507507507507
Chemokine binding protein (Cop-A41L)_VAC_DVX_177660660660660327660660660660660660660660660660
Chemokine binding protein (Cop-A41L)_VAC_DVX_177.1207
Membrane glycoprotein-class I (Cop-A43R) _VAC_DVX_179582582582582582582582582582582582585b582585b591
Unknown (MVA-156R)_VAC_DVX_180237237237237
Thymidylate kinase (Cop-A48R)_VAC_DVX_185615615615615615615615615615615615684b615615684b
Putative phosphotransferase (Cop-A49R)_VAC_DVX_186432432432432432432432432432432432489b489b489b489b
Unknown (Cop-A51R) VAC_DVX_188987210210993210210210210237210216993210210210
Unknown (Cop-A51R) VAC_DVX_188.1801801801801801801759801807801801801
TNF receptor (CrmC) (Cop-A53R)_VAC_DVX_190312312561396246399399561561312399561561399558
TNF receptor (CrmC) VAC_DVX_190.1270
Hemagglutinin (Cop-A56R)_VAC_DVX_192930930933930933930930930930930930930933930930
Schlafen (Cop-B2R)_VAC_DVX_196795795795795795795795795786795786795801375801
Ankyrin-like protein (Cop-B6R)_VAC_DVX_199522522504522522504504522522522522522522522522
Kelch-like (CPV-GRI-B9R) (Cop-B10R)_VAC_DVX_203501501501501501501501501501480501501501501501
Unknown (Cop-B11R)_VAC_DVX_204219255255255255255255255273255279261219285
Serpin (SPI-2) (Cop-B13R)_VAC_DVX_206381381381381381381381381297381381381381381381
IL-1-beta-receptor (Cop-B16R)_VAC_DVX_2099819819819819819819811,002981981981981981981981
Unknown (Cop-B17L)_VAC_DVX_2101,0231,0231,0231,0231,0231,0231,0231,0231,0231,0231,0231,0231,0231,023
Ankyrin (Cop-B18R)_VAC_DVX_2111,7251,7251,7251,7251,7251,7251,7251,7251,7251,7251,7251,7251,7251,725
IFN-alpha/beta-receptor (Cop-B19R)_VAC_DVX_2127987987987981,0627987987987981,0621,0627987981,056
Ankyrin (Bang-B18R) (Cop-B20R)_VAC_DVX_2131,6501,6502,3761,650
Kelch-like (EV-M-167)_VAC_DVX_214243
Kelch-like (EV-M-167)_VAC_DVX_215945
Kelch-like (EV-M-167)_VAC_DVX_216405405405405
TIR paralog DVX_217/026276276276276276276276276276
TIR paralog DVX_218/025168168168168171171168168171168
TIR paralog DVX_219/024216216216216216216216216216
TIR paralog DVX_220/023252252252252252234252252234234
TIR paralog DVX_221/022513894513513894408498513408408
TIR paralog DVX_222/021522522522540522522429429
TIR paralog DVX_223/020273273273273273273273273273273
TIR paralog DVX_224/019381381375375381381381381381375
TIR paralog DVX_225/018189189189189189189189189189189
TIR paralog DVX_226/017252201201201252252273201201252252
TIR paralog DVX_227/015996996996996996996996996996996996
TIR paralog DVX_228/014423423423423426426426426426423420
TIR paralog DVX_229/0131,0741,0741,0741,0741,0741,0741,0741,0741,0741,0741,0741,0621,0741,0741,074
  • a The table shows only genes that differ in some manner between different clones and ORFs of ≥50 amino acids. TIR paralogs DVX_230/012, DVX_231/011, DVX_232/010, DVX_233/009, DVX_234/008, DVX_235/007, DVX_236/006, DVX_237/005, DVX_238/004, DVX_239/003, DVX_240/002, and DVX_241/001 have the same arrangement as the respective left TIR.

  • b The start codon is probably assigned incorrectly. The real gene length is probably identical to that of other “shorter” genes.

  • c TLR, Toll-like receptor.

  • d Ribonucleotide red. large sub., large subunit of ribonucleotide reductase.