Table 4.

Susceptibility of reconstituted WT virus and constructed UL56 mutant viruses to AIC246, approved polymerase inhibitors, and drugs targeting the viral terminase

DrugEC50a (μM) for:RIb
RV-HGRV-HG UL56-L241PRV-HG UL56-R369S
AIC2460.0029 ± 0.0010.47 ± 0.060.11 ± 0.04160, 38
GCVc1.79 ± 0.761.72 ± 0.362.02 ± 1.39
CDVc0.22 ± 0.150.16 ± 0.020.15 ± 0.06
FOSc113 ± 5776 ± 3456 ± 20
BDCRBd0.32 ± 0.120.29 ± 0.020.20 ± 0.03
Bay38-4766d0.36 ± 0.070.27 ± 0.080.52 ± 0.13
  • a EC50s were determined by the fluorescence reduction assay. Data are means from at least three independent experiments and are expressed with standard deviations.

  • b The resistance index (RI) is the EC50 for the indicated RV-HG mutant divided by the EC50 of the RV-HG WT. RI values given are for L241P and R369S mutations, respectively. —, not applicable.

  • c Approved polymerase inhibitor.

  • d Discontinued terminase inhibitor.