Table 1.

Classification of rats exhibiting various degrees of HSE

ClassificationaNo. of rats (%)No. of rats showing neurological symptom ofb:Pathological change scoreGFP-positive region scorefHSV-1-positive region scoref
MonoplegiaQuadriplegiaSeizureTissue damagecCD3-positive cellsdCD68-positive cellse
Severe17 (27)11511+++++++++
Mild14 (22.2)774++++++++++++++
Survived24 (38.1)2200++++
Healthy8 (12.7)000
Total63
  • a HSV-1-infected rats were classified as follows: severe, which died within 5 days after showing symptoms of severe encephalitis; mild, which died between 5 and 7 days after showing symptoms of mild encephalitis; survived, which recovered after transient encephalitis; and healthy, which did not display HSE symptoms.

  • b The number of rats exhibiting neurological symptoms such as monoplegia, quadriplegia, and seizures.

  • c The percentage of damaged areas including cell loss, degeneration of cells, and/or hemorrhaging in the coronal section is presented as follows: −, <5%; +, 5 to 10%; ++, >15%.

  • d The number of CD3-positive cells in one infected area of the coronal section was quantified and is presented as follows: −, not detected; +, <5 cells; ++, 5 to 20 cells; +++, >20 cells.

  • e The number of CD68-positive cells in one infected area of the coronal section was quantified and is presented as follows: −, not detected; +, <5 cells; ++, 5 to 50 cells; +++, >50 cells.

  • f The percentage of GFP- or HSV-1-positive areas in the coronal section is presented as follows: −, not detected; +, <5%; ++, 5 to 20%; +++, >20%.