Table 1.

Cell cycle distribution of GFP- and EYFP-expressing C33A cells

Transfected plasmid(s)a% cells
G0/G1SG2/MIntra-S partitioningb
EML
Mock325315472626
GFP523414562124
GFP-HPV31 E1987485141
GFP-HPV11 E13361775187
GFP-BPV1 E13955676185
GFP-HPV16 E1 WT1681378175
GFP-HPV16 E1 ATPase434414612316
EYFP434314473023
EYFP-31E1 1-6292472475196
EYFP-31E1 1-159494011482528
EYFP-31E1 1-332494011503020
EYFP-NLS463915442828
EYFP-NLS-31E1 160-332454312472826
EYFP-NLS-31E1 160-62937594662212
EYFP-NLS-31E1 332-629454411452727
EYFP384814542521
EYFP-31E1 WT2074677167
EYFP-31E1 OBD384913532422
EYFP-31E1 ATPase37559642215
EYFP-31E1 NES187578875
EYFP-31E1 NES/OBD444412552520
EYFP-31E1 NES/ATPase404416612514
  • a Five thousand cells positively stained with Hoechst stain or doubly stained with Hoechst stain and EYFP/GFP were selected for mock- and EYFP/GFP-transfected cells, respectively.

  • b The percentage of cells estimated to be in the early (E), mid (M)-, or late (L) S phase according to the Modfit software program.