Table 2.

Properties of NS4B mutantsa

MutantReplicationVirus productionHeterotypic interactionMembrane alterationProtein stability
R193A++++NDND
E199A++++Regular++
G200A+++++NDND
W204ANDNDND
N206ANDNDND
R207ANDNDND
I209VNDNDND
F211ANDAberrant++
L208F + F211A+ND++Regular++
F211A + T221N+ND++Regular++
R214ANDAberrant
R192G + R214A+ND++Regular
H217V+++++++Regular
P220ANDAberrant
G200E + P220A+ND++Regular
P220A + I253M+ND++Regular
H222ANDNDND
Y223ANDNDND
E226ANDAberrant++
G200S + E226A+ND++Regular++
A201E + E226AND+Aberrant++
A201E + E226A + NS5A K139E+ND+Regular++
H250A++++++NDND
  • a +++, ∼10 times more than the wild type (wt); ++, same as the wt; +, ∼5 times less than the wt; -, at least 10 times less than the wt, significantly reduced (heterotypic interaction). ND, not determined.