Table 1.

Pseudoreversions selected with replication-defective NS4B mutantsa

Primary mutationPseudoreversionNucleotide exchangeb
F211AL208FCTT→TTT
T221NACT→AAT
±NS5A K139EAAA→GAAc
R214AR192GCGC→GGC
P220AG200EGGC→GAA
I253MATA→ATG
E226AG200SGGC→AGC
A201EGCG→GAG
+NS5A K139EAAA→GAAd
  • a Primary NS4B mutations and corresponding pseudoreversions are given.

  • b That is, the nucleotide substitution detected in the cloned cDNA.

  • c Mutation detected in a fraction of analyzed DNA clones.

  • d Mutation observed in all analyzed DNA clones.