Table 1.

Fitness and yields for the ancestor (wild-type), intermediary, evolved, and mh3dr(D)D34G strains in cell lines expressing exogenous external scaffolding protein genes

Exogenous D genebFitness (doublings/h)a (yield [no. of virions/h/input virus]) of strainc
Wild typeIntermediary strainEvolved strainmh3dr(D)D34G strain
None11.5 ± 0.15 (2,896)5.7 ± 0.80 (52)5.1 ± 0.50 (34)9.2 ± 0.67 (588)
WT13.1 ± 0.32 (8,780)7.2 ± 0.37 (147)7.0 ± 0.56 (128)9.8 ± 0.44 (891)
G61D−6.9 ± 0.32 (0.008)6.2 ± 0.30 (74)11.6 ± 0.21 (3104)−4.5 ± 0.57 (0.044)
G61K−1.5 ± 0.26 (0.354)−1.6 ± 0.37 (0.330)2.4 ± 0.75 (5)−4.1 ± 0.15 (0.058)
D34G8.9 ± 1.1 (478)6.1 ± 1.0 (69)8.3 ± 0.12 (315)9.3 ± 0.78 (630)
D34G/G61D−8.2 ± 0.43 (0.003)3.1 ± 2.4 (9)6.9 ± 0.66 (119)−3.4 ± 0.36 (0.094)
  • a Fitness values are means ± standard deviations.

  • b The expressed D genes have the D34G mutation, an Asp→Gly codon, at position 34 and/or a mutation in glycine codon 61 (G61). The letter after “G61” indicates the amino acid substitution.

  • c The genotypes of the strains are described in the text.